CONFOCOR
3
ConfoCor 3
Acquisition
Carl Zeiss
02/2010 M60-1-0025
e
39
The system will display in the
Calculated Duration
box the total time of the experiment taken into
account measurement times, kinetic data points and the time the system needs for mechanical switches.
The start of the single data points can be retrieved from the
Save Info
panel.
The duration of the kinetic measurement will be displayed in the
Calculated Duration
display box
and in the
Time
selection area.
9.7.1.3
Positions
In the
Positions
panel you can select the carrier
and the sample or laser position by the stage or by
the scanners.
•
Click on the
Position
tab to open the
Position
panel (Fig. 43). Click again to close the panel.
If no carrier was adjusted in the
Carrier
Position
tool, no carrier will be displayed.
You can omit the adjustment if you work
with cells in a single chamber.
Carriers will be schematically displayed with
rows indicated by capital Arabic letters and
columns by numbers (Fig. 44).
Stage control
•
Activate the
XY Stage
control tab to activate stage positioning.
•
Move the stage in the appropriate position by clicking on the arrow keys of the
Compass Rose
. Each
mouse click moves the microscope stage in the appropriate direction by one step.
•
Set the required step width via the
Step-size (µm)
the input box or by using its scroll arrows. The
travel speed of the microscope stage can be set to steps
1
,
2
and
3
. Step 2 is preferably used for
standard positioning. Step 1 (slow speed) should only be used for very precise positioning, since
positioning for longer paths requires more time. Step 3 is suitable if large vessels are used, since
precise center positioning only plays a minor role in such cases.
•
The current x and y positions are displayed in the
x-Position
and
y-Position
input boxes. You can use
the input boxes to change the position by typing in a number or using the scroll arrows. The next click
on one of the compass rose arrows will bring the stage or to that position. The next click will move the
stage or scanner with the current speed and step sizes in the indicated direction.
Fig. 43
Current Positions panel
