CONFOCOR
3
ConfoCor 3
Acquisition
Carl Zeiss
02/2010 M60-1-0025
e
43
(2) Sample
Carrier
The
Sample Carrier
is used to approach multiple
wells sequentially in an automatic mode.
•
Click on the
Sample
Carrier
tab to open the
Sample Carrier
panel (Fig. 45).
Note, the scheme of the carrier is only
visible, if it was adjusted in the
Carrier
Position
panel.
•
Select the working direction from the
Working
Direction
pull down menu (Fig. 46).
Single Position
Only a single well can be activated. The scanning
stage will move automatically to the activated well.
This corresponds to
Current Positions
for sample
carriers.
•
To select the chamber you want to take the
measurement in (only one is selectable) click on
the one you want to use. The motorized stage
moves the selected chamber to the defined
position under the objective. If no scanning
stage is available, positioning has to be done
manually.
•
If you want to move to other positions within a
well, you can do so by imaging the solution and
define positions in
LSM image
.
In
Sample Carrier
only the
X,Y Stage
tab is
active.
•
Select one of the three speeds from the
Speed
Settings
drop down box.
•
Select the step size from the
Step Size (µm)
scroll menu (or the input box) with the scroll
arrows or numerically.
•
The current position will be indicated in the
x-
Position
and
y-Position
selection boxes.
Note, that before clicking on a chamber you
have to orient the carrier. For a non-oriented
carrier, all chambers are displayed in grey.
Please orient first before you select a
position. After orientation, the selected well
will appear blue.
Fig. 45
Sample Carrier panel
Fig. 46
Working direction drop down menu
