CONFOCOR
3
Carl Zeiss
Acquisition
ConfoCor 3
46 M60-1-0025
e
02/2010
•
Move the cursor to the desired position in the
scan image (drag and drop). Press the
Add Pos
button to add the position marked with the
crossline to the
Positions
list (Fig. 48). A
crossline is set at the selected cursor position in
the image (Fig. 49). To select further positions
click at the appropriate position in the image
and then on
Add Pos
. If more than one
position is selected the crosslines are getting
current numbers. The Position list s displays the
numbers and coordinates of the positions in the
Number
,
x (µm)
,
y (µm)
and
z (µm)
display
boxes.
In a Z-stack, you can also vary the Z-plane in
which measurements are to be performed. For
this purpose, select the required Z slice in the
stack or set the Z value via the focusing drive of
the microscope.
Please note, if the
Scanner
button is
pressed, the scanner will be positioned to
the high-lighted position if the
Count Rate
action button is pressed. If the
X,Y Stage
button is pressed, the stage will not be po-
sitioned to the high-lighted position. Only
after triggering a measurement the stage will
move to the indicated position and will go
back to the initial position after the end of
the measurement. If you want to obtain the
count rate by pressing the
Count Rate
button at the indicated position using the
X,Y Stage
control, you have to start a
measurement beforehand.
Please note, that positioning is only valid for
the active (and stored) scanned image. If you
have different images open, be sure to use
the one corresponding to your present
sample.
•
Press the
Del Pos
button to remove selected (highlighted) positions from the
Positions
list and the
corresponding crossline in the scan image.
•
Pres the
Del List
button to remove all positions and all crosslines in the scan image.
•
Press the
Mark Pos
button to fix the crosslines as overlay elements in the scan image (Fig. 49). The
crosslines will also stay visible in the scan image if the
Select
button is deactivated. If you call up an
image later you have to activate the overlay function and a line drawing element to display the
crosslines.
Please note, positioning is performed by scanning mirrors if the
Scanner
button or by the scanning
stage, if the
XY Stage
button is pressed. If more than one position was selected, measurement is
only taken at the highlighted position if the
Snap
action button is pressed. To approach all listed
positions sequentially the
Start ConfoCor Experiment
action button must be pressed.
Fig. 48
Position list with 3 positions
selected
Fig. 49
LSM scan image with 3 selected
positions