CONFOCOR
3
Carl Zeiss
Processing
ConfoCor 3
48 M60-1-0025
e
02/2010
•
Click on the arrow of the
Model
drop down
menu to display a list of pre-defined models
and select one of them. This will load the model
for this channel (Fig. 52). The model name will
be shown in the
Model
display box and the
corresponding model will be shown in the
Model
table. The table has the following
entries:
−
Fit range start:
Designates the start value of
the correlation function taken into account
for the fit. You can change the value by
typing in the display box.
−
Fit range end:
Designates the end value of
the correlation function taken into account
for the fit. You can change the value by
typing in the display box.
−
Parameter:
designates the parameter by its
name. You can activate or deactivate the
parameter by ticking / de-ticking the check
box. Only if the parameter is activated it will
be taken into account for the fit.
−
Value
: designates the currently assigned value of the parameter. You can change the value by
typing in the display box.
−
Type
: designates if a parameter is free floating or if its value is fixed or should be used as a start
value. You can choose between these options in the drop down menu by selecting
Free
,
Start
and
Fixed
, respectively.
−
Lower Limit
: defines the lower limit value as a fit parameter; f this value is falls short, the fit is
rejected and another (global) minimum is searched for. You can change the value by typing the
value in the display box. The default value depends on the parameter, but is in most cases 0.
−
Upper Limit
: defines the upper limit value tolerated from a fit; if this value is exceeded, the fit is
rejected and another (global) minimum is searched for. The default value depends on the
parameter.
−
Link
: globally links the parameter. Type
M
,
K
,
P
,
R
and
C
to link the parameter over measurements,
kinetic indices, positions, repetitions and channels, respectively.
•
The
Status
display window gives information on conflicts in the entries. For example, if the structural
parameter was fixed to 5, but its lower limit set to 6, the system will report this conflict.
•
Before saving you can return to the old settings by pressing the
Reset Parameter
button. This will
undo all changes. If the method was saved, the new entries will be the default.
It is generally accepted that non-linear fitting procedures yield more reliable results when the
number of free floating parameters is low. It is recommended to fix parameters which are known
from independent measurements. Good candidates for fixing are diffusion times of the free dye
and the free (i.e. not bound) partner, which can be determined in previous measurements. Another
good candidate is the structural parameter that is an instrumental parameter.
If you press the
Save
button in the
Save Info
panel all changes will be saved.
Fig. 52
Fit panel with a model selected for
Ch1
