CONFOCOR
3
Carl Zeiss
Processing
ConfoCor 3
50 M60-1-0025
e
02/2010
(2) Count
rate
The
Count rate
panel allows you to specify the
binning time used for the count rate trace.
•
Click on the
Count rate
register to open the
Count rate
panel (Fig. 54). Click any other
register to leave the panel.
You can either check the
Automatic
check-box, in
which case dynamic binning will be applied, or you
can de-activate the check box and enter a number
of a constant binning time (in
ms
) in the
Binning
display window. You can either type in values or
use the arrows. Units are displayed at the right of
the display box. The default value of 1 ms can be
selected by pressing the
1
button.
In (automatic) dynamic binning the count race trace will be adjusted to the measurement length.
The count rate trace represents the averaged binned count rate versus measurement time, in other
words photons / second therefore intensity. The bin window in dynamic binning now depends on
the measurement time, whereby the bin window doubles, if 500 values are exceeded. In the first
step, the binning time is 3.2 ms. For the next steps, the binning time (t
r
) becomes t
r
= 3.2ms x 2n.
The measurement time (t
d
), at which the binning time doubles is calculated according to t
d
= 3.2 ms
x 500 x 2n. In automatic binning mode, the
Binning
display window and the
1
default button will
not be accessible.
(3) PCH
In
PCH
panel the binning time used for the photo
counting histogram can be selected.
•
Click on the
PCH
register to open the
PCH
panel (Fig. 55). Click any other register to leave
the panel.
You can either check the
Automatic
check-box, in
which case 32 different binning times will be used,
or you can de-activate the check box and enter a
number of a constant binning time (in µs) in the
Binning
display window. You can either type in
values or use the arrows. Units are displayed at the
right of the display box. The default value of 10 µs
can be selected by pressing the
10
button.
In automatic binning mode, binning starts with a value of 50 ns, which is doubled 32 times. So
binning times are 50 x 2
n
, with n=1 to 32. The histogram with the best dynamic range will be
selected and displayed. In automatic binning mode, the
Binning
display window and the
10
default button will not be accessible.
Fig. 54
Count rate panel
Fig. 55
PCH panel
