CONFOCOR
3
ConfoCor 3
Models
Carl Zeiss
02/2010 M60-1-0025
e
57
The following relations exist:
D
r
d
⋅
=
4
2
ω
τ
for
1
photon
excitation
(5e)
D
r
d
⋅
=
8
2
ω
τ
for
2
photon
excitation
(5f)
r
z
S
ω
ω
=
(5g)
Equations 5e or 5f, dependent on the excitation source, can be used to retrieve
ω
r
; with its knowledge
ω
z
can be calculated from equation 5g.
N can have different meanings in different fit models. For biology, normally the number of diffusing
particles is of interest. In this case, if photo-physical processes (triplet, blinking, stretched
exponentials) are involved, it is recommended to use their normalized forms, since then the number
of molecules correspond directly to the number of diffusing particles. If photo-physical terms are
not normalized, the number measured is the total number of diffusing particles and those
undergoing photo-physical processes.
