CONFOCOR
3
ConfoCor 3
Models
Carl Zeiss
02/2010 M60-1-0025
e
65
a
r
r
a
r
R
e
c
e
c
R
G
r
r
−
⋅
+
⋅
⋅
+
=
−
−
1
)
(
1
)
(
2
,
2
1
,
1
2
1
τ
τ
τ
τ
τ
normalized
(8f)
with R
a
being the amplitude, c
1
and c
2
relative amplitudes, r
1
and r
2
frequency factors and
τ
r,1
and
τ
r,2
the
rotational diffusion times.
R
a
,
τ
r,1
and
τ
r,2
are fitted parameters; c
1
and c
2
as well as r
1
and r
2
are fixed values and must be user
defined.
Rotational frequencies take often the following values:
r
1
1
r
2
10/3
The relative amplitudes dependent on the polarisation of the excitation light and the analyser in the
emission beam path and are as follows:
Ex lin.
pol.
lin. pol.
lin. pol. unpol. unpol.
unpol.
Em parallel perpendicular all
parallel perpendicular all
c
1
80
20/9
860/9 5/9
215/9
20
c
2
64/9 4
4
16/9 1
1
