CONFOCOR
3
ConfoCor 3
Models
Carl Zeiss
02/2010 M60-1-0025
e
67
τ
d,i
are fitted parameters. They can be converted to diffusion coefficients D
i
using formulas 5e or 5f. The
ConfoCor software allows you to directly fit to D
i
values, but in this case the lateral radius
ω
r
has to be
specified as a fixed value.
Please note that in the case of anomalous diffusion the following relations exist:
i
r
i
d
,
2
2
,
Γ
=
ω
τ
α
1
photon
excitation
(8h)
i
r
i
d
,
2
2
,
2
Γ
⋅
=
ω
τ
α
2
photon
excitation
(8i)
with
Γ
representing the transport coefficient of the fractional time dimension.
Please note the following relation between D and G:
1
)
(
−
⋅
Γ
=
α
t
t
D
(8j)
If activating the fitting to the diffusion coefficient in the ConfoCor the
Γ
values have to be calculated
from the D values by the following conversion:
D
⋅
=
Γ
4
(8k)
The
Φ
i
values are fit parameters. They account for different brightness of different components. In
principle, if molecules of different brightness are present, the apparent molecular brightness is defined as
∑
∑
=
=
⋅
⋅
=
3
1
3
1
2
i
i
i
i
i
i
f
f
η
η
η
(8l)
with
η
i
being the brightness of the molecules in kHz or the dimensionless relative brightness values. The
brightness of the species has to be determined beforehand in control experiments.
