CONFOCOR
3
ConfoCor 3
Action Buttons
Carl Zeiss
02/2010 M60-1-0025
e
113
•
Press the
xyz-Scan
ConfoCor action button to
perform a z scan. The z scan is performed and
the measured intensity is displayed in
dependence of the Z position (Fig. 116). The red
line in the diagram shows the actual z position.
Peaks (normally 2 from the two lower and
upper membranes) show levels of high signal
intensity and may correlate to labeled
membranes. Note, that if the glass surface
happens to be in the z-scan range it will also
give a peak. To determine its position, just
perform a z scan at a position where there is no
cell. If the glass reflection contribute so much
that the peaks of the membranes are hidden
due to scaling, you have to shorten the lower
range end. Place the red line at the peak that
corresponds to the membrane to select this
z
position. Note that z-scan is performed
relative to the current position, which will be
assigned to 0.
•
If no clear signal can be detected, or the peak
of interest lies too close to the border, close the
Scan
window and perform a new z scan.
Choose a different z range by modifying start
and end positions to values that lie closer to the
position at which the signal should be expected.
•
If the z position has been selected, close the
Z-
scan
window by clicking on the
Close
button.
•
Press the
Snap
or
Start ConfoCor Experiment
action buttons to perform an FCS
measurement. The FCS measurement is
performed at the same X/Y position as the Z
scan.
Fig. 116
Z scan image
