CONFOCOR
3
ConfoCor 3
Options
Carl Zeiss
02/2010 M60-1-0025
e
121
9.13
Options
In the major tool group window activate the
Maintain
register (Fig. 127). This will display the
Maintain
and
Options
. Open the
Options for
ConfoCor
menu. The menu has four registers:
−
Data,
−
Measure,
−
LSM + ConfoCor,
−
Auto Save.
9.13.1
Data
9.13.1.1
Saving Raw Data During Measure-
ment
If the
Save raw data during measurement
check box is activated (Fig. 128), raw data will be
saved temporarily in a specified directory on disc
(Fig. 129). Each single measurement will be saved
in a single raw data file. The name of the raw data
file is an identifier to the .fcs measurement it
belongs and the channel. This option is used when
access to the raw data is required to analyze the
data in a different way in respect to correlation
and PCH settings or within other programs.
If the check-box is de-activated, no raw data
will be saved.
Temporary raw data will be deleted from the
specified file upon closing the Image FCS
diagram. You will be prompted in the
Save
raw data
dialogue box, if you want to save
the raw data. Press
Yes
to save the raw data
permanently along with the .fcs file in a
specified directory, which can be defined in
the
Save
dialogue box. Press
No
to discard
the raw data. Note that raw data are saved
along with the .fcs measurement file in the same folder. They are attached to the .fcs file via a
relative file path. If you save the same measurement again without the option
Save Raw Data
,
than the raw data files will be reassigned an absolute file path and the connection to the .fcs file is
lost.
Please note, if the
Auto safe
checkbox is ticked in the
Auto Save
submenu, temporary raw data
will be stored permanently and have to be deleted manually. Care has to be taken to avoid disc
space limitations, since raw data files can be huge.
Fig. 127
ConfoCor Options
Fig. 128
ConfoCor Options - Data
Fig. 129
Stored temporary raw data
