18
02/2010
Setting scan averaging
Averaging improves the image by increasing the signal-to-noise ratio. Averaging scans can be carried out
line-by-line or frame-by-frame. Frame averaging helps to reduce photo-bleaching, but does not give quite
as smooth of an image.
•
For averaging, select the
Line
or
Frame
mode in the
Acquisition Mode
tool.
•
Select the number of lines or frames to average.
Adjusting pinhole size
•
Select the
Channels
tool in the
Left Tool Area
.
•
Set the
Pinhole
size to
1 AU
(Airy unit) for best compromise between depth discrimination and
detection efficiency.
Pinhole adjustment changes the
Optical Slice thickness
. When collecting multi-channel images, adjust
the pinholes so that each channel has the same
Optical Slice thickness
. This is important for
colocalization studies.
→
Fig. 21
Channels tool
