02/2010
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Starting a measurement
•
Select the
Acquisition
toolbar.
The
Times
,
Kinetics
and
Position
panels of the
Acquisition
toolbar display the selected
measurement conditions and the positions which are
used for the FCS experiment.
You can change the settings of this panel using the following function elements:
Enter the
Bleach Time
,
Measure Time
and
Repeat Count
into the corres-
ponding input boxes.
Activate / deactivate a kinetic procedure by ticking the
Use Kinetics
check box.
Enter the time distance between measurements, the cycle number and the shape
in the corresponding input boxes.
Select the carrier and the sample or laser position by the stage or by the
scanners.
Press the
New
button to open a new
FCS diagram
into an image container. If a
measurement is triggered, all data are displayed in that window if highlighted.
Press the
Count rate
button to open the Real Time display window for the
detector
Count rate
in all active channels. This allows you to optimize your
experiment by changing the laser power and the pinhole size while monitoring
the count rate.
Press the
xyz-Scan
button to display the current coordinates. You can define
boundaries where a scan is performed with simultaneous acquisition of the count
rate. This allows you, for example, to identify labeled molecules accumulated in
the membrane.
Press the
Snap
button to trigger one measurement at the highlighted or first
defined position.
Press the
Start ConfoCor Experiment
button to trigger a measurement. All
defined positions will be approached consecutively.
Press the
Stop
button to end a measurement. All data accumulated so far will be
available and can be stored.
After measurement completion, the data is displayed in the FCS Correlation diagram within an Image
Container (see Fig. 35).
Fig. 34
The ConfoCor 3 Measure Tool:
Acquisition
