Imaging and Flow Cytometry Core CPOS
LSM 800 SOP
C-5
ver. 2.1 (Updated: 11/13/2020)
iii.
Select desired objective. Put on lens heater by
first unravelling Velcro tape and then re-attach
around the objective lens
. See image for
reference.
iv.
Mock-up on-stage incubator and adjust
motorized stage position to confirm objective
lens is centred with sample frame.
v.
Position the incubator to the right of wire next
to red dot indicator on microscope stage. Wires
should be on the right hand side and gas tubing
should be on your left hand side.
vi.
Slide incubator to the left while gently pushing
downwards.
vii.
Insert live cell sample.
viii.
Add appropriate amount of MQ water via Leuer-
lock capped tubing.
ix.
Replace incubator cover.
x.
Arrange the gas tubing so that they will not be
trapped under condenser.
C
9iv…Before
C
9iv…After
xi.
Return condenser back to above microscope
stage.
****Observe very carefully that the
condenser does not press onto cover of the
incubator. Push back condenser and find
technical assistance. ***
xii.
Close doors on the acrylic box around the
microscope.
C9xi
