ZEISS
5 How to Get the First Picture
5 How to Get the First Picture
Setting transmitted-light brightfield according to KÖHLER (FULL-KÖHLER version only)
Info
The described functions are not applicable for FIX-KÖHLER versions. Refer to the Instruction
Manual for further information.
A
B
C
D
E
1
2
2
3
3
7
4
6
5
Fig. 11: Microscope adjustment in transmitted light brightfield microscopy
Prerequisite
ü
The Microscope is started up and switched on.
ü
The stop is adjusted to prevent the condenser from lifting out the sample.
Procedure
1. Put a high-contrast sample into the specimen holder of the mechanical stage.
2. Adjust the image brightness using the light intensity control
1
on the microscope stand.
3. On condensers with revolver/modulator disks: turn the knurled ring
4
to position
BF
=
brightfield.
4. Bring the 10x objective into position on the nosepiece
7
.
5. Focus the sample using the focus drive
2
.
6. Close the field diaphragm
5
enough to make it visible in the field of view (even if
blurred
A
).
7. Lower the condenser using the knurled knob for vertical adjustment
6
until the edge of
the field diaphragm appears sharp
B
.
8. Center the field diaphragm image
C
with both centering screws
3
on the condenser
carrier.
9. Open the field diaphragm enough to make the edge of the diaphragm disappear from the
field of view
D
.
10. To adjust the aperture (contrast), remove an eyepiece from the tube barrel. Looking
through the barrel adjust the aperture with the adjusting lever of the aperture diaphragm
to the size of approx. 2/3 -4/5 of the diameter of the objective exit pupil
E
.
In most cases this aperture gives the best contrast at almost full resolution and is thus the
best compromise for the human eye.
11. Replace the eyepiece in the tube barrel.
Quick Guide ZEISS Primostar 3 | en-US | Rev. 1 | 415501-7012-101
23
