ZEISS
Start-Up and Operation
Primovert
42
415510-7244-001
12/2014
3.7
Adjusting Transmitted-Light Phase
Contrast
•
First, adjust the microscope as you do for
brightfield.
•
Turn the nosepiece to position the phase-
contrast objective (for Ph 0, Ph 1 or Ph 2) into
the light path.
•
Open the aperture diaphragm completely by
means of the lever (Fig. 33/
3
) on the condenser.
•
Introduce the slider (Fig. 33/
1
) into the
condenser so that the phase stop position
suitable for the objective used (Ph 0, Ph 1 or
Ph 2) is in the light path. Pay attention to the
correct detent position.
•
Adjust the illumination intensity as needed.
Stand with Binocular Tube or Phototube
•
Check the centering of the phase stop
according to the drawing in Fig. 34. To this end,
remove one eyepiece and replace it by the
diopter.
•
Center the phase stop, if necessary (Fig. 34/
A
),
by turning the two adjusting screws (Fig. 33/
2
)
of the corresponding slider position by means
of the two Allen keys SW 1.5 (Fig. 33/
4
) until
the image corresponds to that shown in
Fig. 34/
B
.
•
Afterwards, replace the diopter by the eyepiece
again.
Stand with Monitor
•
Put on the standard phase contrast specimen.
•
Use the two adjusting screws to center the
annular diaphragms on the monitor display,
while taking the following into account:
−
Ensure even monitor display illumination for
the purpose of coarse adjustment.
−
For fine adjustment, set the contrast to
maximum, so that the texture is as bright as
possible, while the background is as dark as
possible.
Fig. 33
Inserting the slider
Fig. 34
Centering the phase stop
