Zeiss Standard 25 ICS, Operating Manual

Page 1: ...Standard 25 ICS Transmitted Light Microscope Operating Manual...

Page 2: ...al is not covered by an update service Unless expressly authorized forwarding and duplication of this document and the utilization and communication of its contents are not permitted Violations will e...

Page 3: ...hnical Data 1 10 2 START UP 2 3 2 1 Unpacking the instrument 2 3 2 2 Attachment of binocular tube 45 20 ICS or binocular phototube 35 20 ICS 2 3 2 3 Screwing in of objectives 2 3 2 Insertion of eyepie...

Page 4: ...167 MT 3 11 3 7 2 Attachment of MC 80 DX microscope camera 35 mm film cassette 3 13 3 8 Attachment of adapters for video cameras 3 14 3 9 Insertion of 8x drawing eyepiece 3 16 4 CARE MAINTENANCE AND...

Page 5: ...and UL directives The microscope meets the requirements of the EC directive 89 336 EC and the EMC legislation of November 9 1992 This operation manual includes information and warnings which must be...

Page 6: ...nding on the model Always disconnect the instrument from the line before opening the instrument before changing the voltage and before changing the fuses When changing the instrument fuses make sure t...

Page 7: ...is by repairing the instrument or by delivering an instrument free of any defect No guarantee is provided for defects caused by natural wear wearing parts in particular The instrument manufacturer is...

Page 8: ...Standard 25 ICS VIII B 40 029 e 01 98 Overall view of the Standard 25 ICS microscope with polarization equipment...

Page 9: ...3 1 2 Instrument description and main features 1 3 1 3 Microscope configurations and modules 1 4 1 4 Objectives 1 6 1 5 Eyepieces 1 8 1 6 Stage micrometers and eyepiece reticles 1 8 1 7 Technical Data...

Page 10: ...40 029 e 01 98 Fig 1 1 Microscope modules 1 Eyepieces 5 Condenser carrier with condenser 2 Binocular tube 6 Mechanical stage with specimen holder 3 Stand 7 Objective 4 Coaxial coarse and fine drive 8...

Page 11: ...es an optional camera port for photo and video documentation is also available to the user Major instrument features Sturdy and convenient stand in the pyramid design User friendly coaxial coarse and...

Page 12: ...Standard 25 ICS 1 4 B 40 029 e 01 98 1 3 Microscope configurations and modules Fig 1 2 Standard 25 ICS microscope configurations with polarization equipment...

Page 13: ...10x Ph 1 40x Ph 2 and 100x Ph 3 and phototube 490868 9804 490868 9904 Modules 1 1 Standard 25 ICS microscope stand with mechanical stage 75x30 R 5 position nosepiece W 0 8 including integrated illumi...

Page 14: ...ength and not with instruments the objectives of which are marked with 160 as their mechanical tube length The exact observance of the cover slip thickness of 0 17 mm is all the more necessary the hig...

Page 15: ...4 441031 A Plan 20x 0 45 Ph 2 0 53 0 17 441041 A Plan 40x 0 65 Ph 2 0 43 0 17 441051 A Plan 100x 1 25 Oil Ph 3 0 22 0 17 441081 Achrostigmat Transm light brightfield Achrostigmat 20x 0 45 1 6 0 17 44...

Page 16: ...ired eyecups for the eyepieces can be ordered under the Cat No 444801 1 6 Stage micrometers and eyepiece reticles Measuring and counting using the microscope requires stage micrometers and eyepiece re...

Page 17: ...6 mm area 12 5x12 5 mm divided in fields of 5x5 or 10x10 474068 Photo reticle MC 2 5x d 26 mm for 35 mm photography with an additional magnification of 2 5x or for large format photography with a 10x...

Page 18: ...tion max 100 Operation Permissible ambient temperature 10 to 35 C Permissible relative humidity without condensation max 85 Altitude max 2000 m Atmospheric pressure 800 hPa to 1060 hPa 4 Operating dat...

Page 19: ...r 20 E Pl 10x 20 Br and E Pl 10x 20 Br foc Specimen stage mechanical stage 75x30 R with ceramic surface Dimensions width x depth 160 x 140 mm Travel range width x depth 75 x 30 mm Specimen holder with...

Page 20: ...ximum field number 20 interpupillary distance can be set between 55 and 75 mm viewing angle 35 viewing height approx 430 mm visual port tube factor 1x camera video port tube factor 1x camera video por...

Page 21: ...2 7 Attachment of condensers 2 7 2 7 1 Attach condenser 0 9 Z 2 7 2 7 2 Attach condenser II Z 0 9 Ph1 2 3 and D 0 6 0 9 2 7 2 8 Connecting the instrument to the line 2 8 List of illustrations Fig 2 1...

Page 22: ...Standard 25 ICS 2 2 B 40 029 e 01 98...

Page 23: ...eaded screwdriver 2 1 4 Remove dust covers 2 1 1 from tube underside and the dovetail mount on the stand Hold the binocular tube 2 1 3 or the binocular phototube 2 1 2 in a slightly inclined position...

Page 24: ...1 6 The slight image shift caused by the additional path through the glass is taken into account on the diopter scale by the fact that the zero point position is indicated not by the white dot W 2 4 W...

Page 25: ...y looking through the eyepiece with reticle When the image and the eypiece reticle are in focus in the above eyepiece focus the image for the second eye via the focusing eyelens of the second eyepiece...

Page 26: ...lternative it is also possible to use the specimen holder with sprig clip R 473448 2 6 2 or the specimen holder for one handed operation 453548 2 6 3 Here the microscope slide 2 6 7 is inserted into t...

Page 27: ...ition The Ph 2 phase stop carrier 2 7 4 must be mounted as follows Screw locking screw 2 7 6 into the left drilled hole 2 7 7 on the underside of the condenser carrier The locking screw keeps the Ph 2...

Page 28: ...al and make sure to inform the nearest CZ agency or CZ service agency Connect the line cable with connector 2 9 4 to the instrument socket 2 9 3 and connect the earth contact plug 2 9 5 to the line Sw...

Page 29: ...ent of adapters for video cameras 3 14 3 9 Insertion of 8x drawing eyepiece 3 16 List of illustrations Fig 3 1 Switch on the instrument 3 3 Fig 3 2 Insertion of filters 3 3 Fig 3 3 Setting of brightfi...

Page 30: ...Standard 25 ICS 3 2 B 40 029 e 01 98...

Page 31: ...us field diaphragm interference wide band filter green 32x4 for contrast enhancement in b w photography of stained sections and for phase contrast CB 3 conversion filter 32x2 to generate the correct c...

Page 32: ...0 R 3 3 8 Swing 10x objective into beam path via knurled ring 3 3 9 of the nosepiece Look throught the fixed eyepiece of the binocular tube 3 3 1 first and focus on the object via the focusing drive 3...

Page 33: ...entering screws 3 3 7 3 5 C Open the luminous field diaphragm 3 3 3 until it just disappears behind the edge of the field of view 3 5 D Depending on which specimen is used the image contrast can be ad...

Page 34: ...denser 0 9 Z 3 6 9 and Ph 2 phase stop carrier 3 6 7 phase contrast objectives labelled Ph 2 3 6 11 suitable also for transmitted light brightfield Settings Place unstained specimen on the mechanical...

Page 35: ...ld first by having the turret disk 3 8 5 click stop in position J Swing in the condenser front lens 3 8 2 via lever 3 8 4 Reduce luminous field diaphragm 3 6 5 focus it using the condenser center it a...

Page 36: ...75 1 0 Higher objective magnifications or apertures require objectives with an integrated iris aperture As described in chapter 2 the microscope is ready for operation and switched on according to sec...

Page 37: ...enable the function to be performed the sliders must be pushed in Settings First set a suitable object in brightfield as described in section 3 2 Swing the polarizer 3 9 3 in the beam path If only the...

Page 38: ...of the stage micrometer correspond to exactly 100 increments of the crossline micrometer as in Fig 3 10 the resulting scale value k for the used objective eyepiece reticle combination A Plan 10x 0 25...

Page 39: ...g 3 11 2 and fix the cable release 3 11 1 if required Loosen the three hexagonal screws 3 11 6 remove the dust cover 3 11 8 from the camera tube 3 11 5 and insert the premounted unit A in the camera t...

Page 40: ...bayonet 416010 T2 adapter for OLYMPUS OM OM bayonet 416002 T2 adapter for MINOLTA SR bayonet 416003 T2 adapter for CANON FD bayonet 416004 T2 adapter for NIKON F bayonet 416009 T2 adapter for PENTAX...

Page 41: ...body in such a way that the contact pins firmly engage in the relevant sockets Pull out pushrod 3 12 8 completely for photomicrography When artificial light color reversal film is used the CB 3 conve...

Page 42: ...cameras and three chip color CCD cameras to the camera tube of the Standard 25 ICS Tube Adapter Cameras 456105 60 C 2 3 1 0x 456107 60 C 2 3 0 63x 456106 60 C 1 2 0 5x 456119 60 C 1 3 3CCD 0 5x 456108...

Page 43: ...mount thread into the video camera Insert video adapter or video zoom adapter in ENG 2 2 or ENG bayonet of the video camera and clamp it tight Insert premounted unit video camera with video adapter or...

Page 44: ...t brightfield as described in section 3 2 Settings Remove one eyepiece 3 13 1 and insert the 8x drawing eyepiece 3 13 3 in the binocular phototube 35 20 ICS 3 13 2 instead of it Swing 8x drawing eyepi...

Page 45: ...aintenance of the instrument 4 3 4 2 Troubleshooting 4 4 4 2 1 Changing the fuses 4 4 4 2 2 Changing the 6 V 20 W halogen lamp 4 5 4 2 3 Troubleshooting table 4 6 4 3 Table of spares wearing parts and...

Page 46: ...Standard 25 ICS 4 2 B 40 029 e 01 98...

Page 47: ...isten the cloths with petroleum ether Clean the front lenses of the objectives using petroleum ether but do not use alcohol When using the Standard 25 ICS in humid climatic zones proceed as follows St...

Page 48: ...he line if required also disconnet flat plug 4 1 3 from the instrument connector 4 1 2 Check line cable and instrument plugs 4 1 3 and 4 and exchange them if required Press marked lock 4 1 5 in the di...

Page 49: ...ble Fold down cover 4 2 2 and pull out defective halogen lamp 4 2 1 Use the protective film or a clean paper cloth to hold the new 6 V 20 W halogen lamp and insert both lamp pins carefully into the re...

Page 50: ...see pages 3 11 and 3 13 Nosepiece with objective not switched to stop position Switch nosepiece with objective in stop position Condenser not set correctly Set condenser correctly adjustment centerin...

Page 51: ...Air bubbles in the immersion oil Apply oil again to remove the bubbles Immersion oil at the front lens of a dry objective Clean the front lens of the dry objective see page 4 3 Corr Ring is not set to...

Page 52: ...denser lens 0 9 correctly or switch it off see pages 3 4 3 5 Aperture diaphragm opening too small Set aperture diaphragm opening in accordance with the 2 3 rule or depending on the specimen features s...

Page 53: ...ife of 6 V 20 W halogen lamp Replace 6 V 20 W halogen lamp see page 4 5 Incorrectly installed or broken line cable Correctly connect line cable or replace it see page 4 4 The pins of the 6 V 20 W halo...

Page 54: ...erload protection for the integrated power supply Light filters interference wide band filter green d 32x4 Interference band filter green 546 d 32x3 CB 12 conversion filter d 32x2 CB 6 conversion filt...

Page 55: ...25 ICS may only be performed by Carl Zeiss service staff or specially authorized personnel To ensure the optimum setting and trouble free function of your microscope even for a longer period of time w...

Page 56: ...Standard 25 ICS 4 12 B 40 029 e 01 98...

Page 57: ...Standard 25 ICS B 40 029 e 01 98 A 1 ANNEX List of abbreviations A 3 Physical and technical units A 4 Certification in accordance with DIN EN ISO 9001 DIN EN 46001 A 5 EC conformity declaration A 7...

Page 58: ...Standard 25 ICS A 2 B 40 029 e 01 98...

Page 59: ...electronically legible information e g film speed EG European Community EMV electromagnetic compatibility EN European standards ENG Electronic News Gathering E PL name of eyepiece type with aspheric...

Page 60: ...ow blow fuse type TV television T2 Adapter standard adapter for 35 mm cameras UL Underwriters Laboratories US test authority VObj magnification of the objective VDE association of German electrotechni...

Page 61: ...Click below to find more Mipaper at www lcis com tw Mipaper at www lcis com tw...