Standard 25
ICS
3-4
B 40-029 e 01/98
3.2
Setting of transmitted-light brightfield for KÖHLER illumination
As described in chapter 2, the microscope is ready
for operation and switched on according to
section 3.1.
If the Ph 2 phase stop carrier (3-3/
4
) is available
on condenser 0.9 Z (3-3/
6
), it should be swung
out of the optical beam path for microscopy in
transmitted-light brightfield.
Furthermore, the following basic settings of the
microscope are required:
First, place a high-contrast specimen with 0.17
mm cover slip on the mechanical stage 75x30 R
(3-3/
8
).
Swing 10x objective into beam path via knurled
ring (3-3/
9
) of the nosepiece.
Look throught the fixed eyepiece of the
binocular tube (3-3/
1
) first and focus on the
object via the focusing drive (3-3/
2
).
Then set the focus for the other eye by turning
the eyelens of the focusing eyepiece.
The eyepiece distance is matched to the
individual interpupillary distance by swinging
the eyepiece tubes symmetrically towards one
another.
Set the interpupillary distance in such a way
that you can see a sharply limited field of view
with both eyes.
The viewing height can be increased (3-4/
A
) or
reduced (3-4/
B
) by turning the entire binocular
tube through 180°.
Fig. 3-3
Setting of brightfield
Fig. 3-4
Setting of interpupillary distance of
the binocular tube
Summary of Contents for Standard 25 ICS
Page 1: ...Standard 25 ICS Transmitted Light Microscope Operating Manual...
Page 22: ...Standard 25 ICS 2 2 B 40 029 e 01 98...
Page 30: ...Standard 25 ICS 3 2 B 40 029 e 01 98...
Page 46: ...Standard 25 ICS 4 2 B 40 029 e 01 98...
Page 56: ...Standard 25 ICS 4 12 B 40 029 e 01 98...
Page 58: ...Standard 25 ICS A 2 B 40 029 e 01 98...
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