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EXC-120 Microscope Series
Phase Contrast Supplement
Alignment of the Phase Contrast Components
Underside View of Phase Turret,
Variable Aperture Diaphragm
and 4 Annuli Holders, Adjustable (X-Y)
Components
Phase contrast consists of two components for each phase
contrast objective installed on the microscope:
Phase Retarder Ring
inside each
Ph
objective. This gray
Phase ring is observed using the focusable
Phase Centering
Telescope
supplied with the system. In the following
instruction you will be advised when to substitute the
telescope for one of the microscope’s eyepieces
Annulus Ring
in the condenser. There is a different
annulus for each objective—one ring corresponding to each
Ph objective. Its purpose is to shape the incoming light into
a hollow cylinder. The annuli are mounted in holders that are
individually movable in an X-Y manner— so as to be able to
align the image of each annulus with the gray phase ring in
each objective.
Front Turret Installed and Positioned
Currently in Optic Axis
Installation
Install the Ph objectives
on the microscope’s nosepiece
starting with the 10x, and then rotate the nosepiece clockwise
and install the 20x, 40x and 100xR oil objectives in numerical
order.
Install the Phase Contrast Condenser/Turret
into the
condenser carrier mounting ring and tighten the set screw
with the screwdriver that came with your microscope.
NOTE:
The condenser mounting ring has been pre-centered
at the factory.
Select
the
B
for
brightfield
position on the Condenser/Turret
by rotating it clockwise until the B clicks into place as shown.
Rotate the nosepiece and
bring the 10x objective
into
position.
Raise the Condenser/Turret
to its highest position.
Place a familiar “prepared, stained specimen”
on the
stage and focus the 10x objective on this specimen.
10x Annulus
20x Annulus
40x Annulus
100x Annulus
