Accu-Scope EXI-600, Manual

Page 1: ...MANUAL EXI 600 INVERTED RESEARCH MICROSCOPE SERIES 73 Mall Drive Commack NY 11725 631 864 1000 P 631 543 8900 F www accu scope com info accu scope com ...

Page 2: ...11 Fluorescence light source optional 19 5 2 12 UV shield optional only needed for fluorescence 19 5 2 13 Fluorescence sliders optional fluorescence only 20 5 2 14 Power supply 21 5 2 15 Camera adapter 22 6 OPERATION 23 6 1 COMPONENT ADJUSTMENTS 23 6 1 1 Trinocular observation tube adjustments 23 6 1 2 Coarse Fine focus 24 6 1 3 Condenser turret Components 25 6 1 4 Kohler Illumination Aligning the...

Page 3: ... 6 2 2 Reflected Light Methods 37 6 3 Microscopy Imaging 38 7 TECHNICAL SPECIFICATION 39 7 1 Main technical specification 39 7 2 Objective specifications 40 8 EXI 600 CONFIGURATION DIAGRAM 41 9 EXI 600 FLUORESCENCE CONFIGURATION DIAGRAM 42 10 TROUBLESHOOTING 43 10 1 Optical 43 10 2 Electrical 44 10 3 MICROSCOPY IMAGING 45 11 MAINTENANCE 46 12 LIMITED MICROSCOPE WARRANTY 47 ...

Page 4: ...lar attention to the sections where these symbols appear Symbol Meaning WARNING Symbol indicates that ignoring this alert may result in personal injury or even death NOTICE Symbol indicates that ignoring this alert may result in personal injury or loss of property Meaning of symbols found on the instrument Symbol Meaning Ground fault terminal CAUTION Hot surface CAUTION Indicates hazard to the use...

Page 5: ...roscope hold the transport handles tightly and raise the microscope above the bench before moving it Do not drag push the microscope across the bench 3 If liquid e g culture medium water etc drips or is spilled onto the stage objective or observation tube immediately turn off the power supply and wipe off the solution to prevent further damage 4 When operating the lamp house can be considerably ho...

Page 6: ...y the included dedicated cables 8 Install the instrument in a location away from any acidic gasses alkaline agents organic solvents and any harmful substances that may damage the instrument or harm the operator A grounding 3 pin plug is provided and recommended for the instrument s safe operation Use of any other adapter plug will decrease the performance of the instrument s safety features and po...

Page 7: ...illumination shutter On Off 11 Reflected illumination shutter Open Closed 12 Right coarse fine focusing knob 13 Magnification changer turret 14 Right camera port 15 X Y axis controls of sample stage 16 Reflected light fluorescence filter cube turret 17 Reflected light field diaphragm 18 Reflected light aperture diaphragm 19 3 hole filter slider 20 Objective nosepiece 21 Sample stage 22 Condenser t...

Page 8: ...d analysis such as brightfield standard phase contrast differential interference contrast DIC and fluorescence and is an ideal platform for integration with 3rd party accessories such as LED based fluorescence illuminators digital imaging cameras motorized stages motorized filter wheels motorized focus and manipulators Please contact an ACCU SCOPE salesperson to discuss your application and what p...

Page 9: ...lamp house stage and other accessories such as filters DIC blocks dust proof shield tools optional and user manual Optional and 3rd party accessories may be shipped in packages separate from the main packaging 1 Open all the packaging check the goods and confirm the accordance with the products you purchased Keep all dust caps covers tools and user manuals in safe place 2 Remove the transport hand...

Page 10: ...ot discard keep in safe place Align and place the dovetail of the trinocular tube into the receptacle on the frame Tighten the 2mm hex screw to secure the trinocular tube onto the frame Hold the trinocular tube securely to avoid it falling during installation A slot is built into the mainframe through which the 2mm hex screwdriver will fit to tighten the 2mm hex screw Transport handles Mainframe F...

Page 11: ...frame These screws will be used again momentarily Hold the pillar above the mainframe with the base of the pillar directly above the mounting position Align the guide pins with the slots in the frame Slowly slide the pillar down into the slots The pillar is not yet secured but will not fall over Secure the pillar onto the frame using the same 4 hex screws from above When installed properly the pil...

Page 12: ...e already installed The instructions below may be useful for maintenance or replacement Insert the dovetail of the lamp house into the socket and ensure the dovetail is fully inserted The lamphouse should be parallel to the bench Use a 2mm hex screwdriver and tighten the locking screw to secure the lamphouse to the socket Clip the power cord into the plastic clips on the back of the pillar Socket ...

Page 13: ...stall the objectives from low magnification to high beginning with the lowest position number on the nosepiece If installing different types of objectives e g brightfield and phase contrast install all of one type before installing the next Remove a dust plug from the nosepiece Screw an objective into the threaded opening Note the position number on the nosepiece that corresponds to the objective ...

Page 14: ...e various specimen holders such as microscope slides and multiwell plates Align the stage insert close to the spring on the edge of the stage Press the stage insert into the stage opening until it is level and flush with the stage surface Only one stage insert can be installed at a time and this procedure allows for easy exchange of inserts based on need Stage insert Spring ...

Page 15: ...ndenser onto the transmitted light illumination pillar Align the guiding pin on the top front edge of the dovetail with the alignment groove on the front of the condenser carrier Slide the dovetail of the condenser into the condenser carrier Ensure the dovetail is completely inserted into the carrier Secure the condenser by tightening the lock screw Condenser centering screw Dovetail Alignment gro...

Page 16: ...he top of the condenser Rotate the condenser turret until either the DIC or DIC II position is centered in the opening Remove the retaining ring if present Set the DIC condenser prism into the opening Be sure to align the groove of the prism with the pin in the opening and that any writing is oriented upright 40X not Ensure that the prism is completely seated in the opening DIC position for 10X ob...

Page 17: ...isengaged the analyzer is completely removed from the optical path Remove the rubber dust plug located just below the objective to be used for DIC observation Insert the DIC nosepiece prism that corresponds to the objective magnification DIC Analyzer Slider in OUT disengaged position DIC Analyzer Slider in IN engaged position Dust plug DIC nosepiece prism DIC nosepiece prism installed ...

Page 18: ... a cube into the metal clip Gently push onto the back of the cube until the top of the wings click into the upper metal clips NOTE When installed correctly there is no filter in the upper face of the filter cube filters occupy the front and bottom positions only in addition to the beamsplitter To insert the cube turret into the frame turn the locking lever down Raise the cover to reveal the locati...

Page 19: ...source optional Align and insert the fluorescence light source adapter dovetail into the receiver on the back of the microscope requires epi fluorescence vertical illumination is already installed Secure the light source adapter by tightening the 2mm hex screw using a 2mm hex screwdriver 5 2 12 UV shield optional only needed for fluorescence NOTE A UV shield is recommended for fluorescence microsc...

Page 20: ...the A slot of the mainframe Detents in the slider help identify the out and in positions Insert the 3 position Neutral Density ND filter slider into the rear most slot to the right of the Aperture iris slider Starting adjustment for fluorescence Set the Field and Aperture sliders to the top fully open positions Using the detents on the ND filter slider push in or pull out the slider until the midd...

Page 21: ...on the back of the microscope The lamp house power connector must be oriented in the 3 o clock position Press in gently to secure the connection To remove simply grasp the power connector and pull straight back from the microscope Connect the power adapter to the power port labeled INPUT on the back of the mainframe Attach the slotted end of the power cord to the power adapter and the plug to a su...

Page 22: ...rientation when threading them together Thread the camera adapter with attached camera onto the side port Orient Camera Image After installing the camera the image to the monitor may be in a different orientation than seen through the eyepieces Loosen the camera adapter locking screws 3 total with a 2mm hex screwdriver Compare the image on the monitor with the image seen through the eyepieces and ...

Page 23: ...until the image in one eyepiece is perfect Adjust the diopter of the other eyepiece until the image through that second eyepiece is good The eyepieces can be adjusted by a maximum of 5 diopters Adjust interpupillary distance While viewing a sample grasp and adjust the eyetubes up or down at the binocular hinge to increase or decrease the interpupillary distance distance between the pupils of the o...

Page 24: ...ent 0 2mm rotation Rotation of the focus knobs away from the operator i e left side focus knobs move in counterclockwise direction moves the objective nosepiece up Rotation of the focus knobs toward the operator i e left side focus knobs move in clockwise direction lowers the nosepiece Focusing knobs are located on both sides of the microscope The left side displays a scale 0 10mm division Fine fo...

Page 25: ...rence Contrast for 10X objective DIC II Differential interference Contrast for 20X 40X 60X objectives Adjust the aperture diaphragm by rotating the condenser diaphragm wheel The condenser aperture diaphragm is adjustable ONLY when the condenser turret is in the B DIC and DIC II positions The aperture is automatically fully open auto iris feature for Ph1 Ph2 and Ph3 L positions and cannot be adjust...

Page 26: ...knobs adjust the condenser position up or down until the bright area seen in the specimen is seen sharply Flat edges of the field diaphragm should become visible Using the condenser centering screws center the bright area in the field of view by screwing in or out the centering screws Open the field diaphragm until the bright area fills most of the field of view Adjust condenser centration again u...

Page 27: ... IN Ph1 Ph2 or Ph3 L positions Turn the condenser turret to the brightfield B position Focus on a specimen Remove one eyepiece If it is too bright reduce illumination intensity so that the leaves of the condenser aperture diaphragm can be clearly distinguished Adjust the condenser diaphragm until the field of view is illuminated approximately 75 open The condenser aperture position is directly rel...

Page 28: ...e Change to the phase contrast objective for which the corresponding phase annulus will be centered Change the condenser turret to the phase annulus position Ph1 Ph2 Ph3 L that corresponds to the objective magnification The condenser diaphragm is fully open automatically Engage the built in Bertrand lens by moving the black knob on the front of the observation tube to the B position While looking ...

Page 29: ...risms BEFORE proceeding with DIC setup perform Köhler illumination per section 6 1 4 above Rotate the condenser turret to the DIC or DIC II position Select an objective with DIC prism that corresponds to the DIC condenser prism selected above i e 10X with DIC 20X or 40X with DIC II Temporarily remove the DIC nosepiece prism Focus on a specimen Engage insert the analyzer fully Loosen slightly the t...

Page 30: ...r wheel has 3 positions with different light splitting conditions 100 eyes 0 camera port 20 eyes 80 right side camera port 0 eyes 100 left side camera port 6 1 8 Magnification changer Rotate the magnification changer to change the magnification for observation Available magnifications 1X Does not alter the magnification of the view 1 5X Increases the magnification by 1 5X Blank no label This posit...

Page 31: ...osition to attenuate fluorescence illumination intensity Position 1 50 Transmission Position 2 100 Transmission Position 3 25 Transmission Detents Field diaphragm Controls the size of the illuminated field Adjust the diaphragm opening by moving the diaphragm iris adjustment lever up to open or down to close The field diaphragm iris can be centered in the light path by adjusting the two centering s...

Page 32: ...iris completely Aperture diaphragm Regulates the intensity of illumination without affecting the size of the field Adjust the diaphragm opening by move the diaphragm iris adjustment lever up to open or down to close The aperture diaphragm is pre centered at the factory and further adjustment should not be necessary Blank slider Ensure that the blank slider is fully inserted into the slot The deten...

Page 33: ...an objective NOP No Object Present Nosepiece position 1 2 3 4 5 6 Fluorescence filter cube turret positions Each of the six positions on the intelligent filter cube turret can be programmed into the LCD panel When in program mode choose the magnification that corresponds to the filter cube in that position of the turret Arrows on the turret indication the position as recognized by the LCD panel Ma...

Page 34: ...y Program the objective positions and fluorescence filter cube positions using the Settings gear icon Press the Settings icon It will change to a bubble appearance Press a button for objective or fluorescence turret position to program Choose from the available options Press another button to program it When finished press the bubble icon to accept the changes and the bubble icon will change back ...

Page 35: ...ce cube turret if installed Choose an open position no filter cube installed 1 Place a specimen on the stage 2 Focus on the specimen using the focus knobs 3 If desired change side port light path selector or trinocular tube upper light path selector to a camera port for imaging 6 2 1 2 Phase contrast observation Instrument configuration Component Position Transmitted illumination shutter On open l...

Page 36: ... Instrument configuration Component Position Transmitted illumination shutter On open light is coming out of the bottom of the condenser Upper light path selector Lower light path selector Condenser turret position DIC DIC II Magnification changer 1X Fluorescence cube turret if installed Choose an open position no filter cube installed Objective s Match the objective with the condenser turret posi...

Page 37: ...nser turret position Any position Condenser is not being used during fluorescence observation It is recommend to tilt the light pillar to the back position to avoid reflection and autofluorescence of the transmitted LED light source Magnification changer 1X Fluorescence cube turret if installed Choose an appropriate filter cube for the specimen Objective s Any as provide appropriate magnification ...

Page 38: ...r Upper selector 50 eyes 50 camera port 0 eyes 100 camera port Bertrand lens selector Upper selector No observation options available Left side port 0 eyes 100 camera port Right side port 20 eyes 80 camera port 1 Focus on specimen 2 Change light path selector to desired camera port Image should now appear on computer monitor or camera display 3 Adjust camera settings per camera manufacturer or sof...

Page 39: ...chromat Modulation contrast 10X 20X 40X S Plan Fluor Focusing Coaxial coarse and fine focus Coarse focusing 2mm rotation Fine focusing 0 2mm rotation Stroke from focal point at stage 7mm up 2mm down Stage Range of movement 135mm X direction 80mm Y direction Illumination 10W LED pre centered continuous variable intensity Condenser Six position rotatable focusable NA 0 55 WD 26mm Operating environme...

Page 40: ...X 0 95 0 11 0 23 0 21 0 17 60X oil 1 40 0 14 0 17 100X oil 1 45 0 13 0 17 BF DIC FL Plan semi APO 4X 0 13 17 0 1 2 10X 0 30 7 4 1 2 20X 0 45 8 0 1 2 40X 0 60 3 3 1 2 60X 0 70 1 9 1 2 PH BF DIC Plan semi APO 4X 0 13 17 0 1 2 10X 0 30 7 4 1 2 20X 0 45 0 2 7 5 8 8 1 2 40X 0 60 0 2 3 0 4 4 1 2 60X 0 70 0 1 1 3 1 8 2 6 1 2 MC BF sPlan FL 10X 0 25 10 5 1 2 20X 0 45 0 2 8 1 7 0 40X 0 60 0 2 3 6 2 8 BF Br...

Page 41: ...11725 631 864 1000 www accu scope com 41 8 EXI 600 CONFIGURATION DIAGRAM 1 Main microscope frame 2 Trinocular observation tube 3 Eyepiece 4 Camera adapter 5 Objective 6 DIC nosepiece prism 7 Stage 8 Condenser 9 LED lamp house 10 Transmitted illumination pillar 11 UV shield ...

Page 42: ... scope com 42 9 EXI 600 FLUORESCENCE CONFIGURATION DIAGRAM 1 Main microscope frame 2 Fluorescence filter cube turret 3 Fluorescence filter cube 4 Analyzer slot blank slider 5 Field diaphragm slider 6 Blank slider 7 Aperture diaphragm slider 8 Neutral Density filter slider 9 Fluorescence light source ...

Page 43: ... engaged Rotate the condenser turret until it sets completely into a position as felt by the detent Dirt or dust in the field of view Dirt or dust in the light path e g on the eyepiece condenser objective collector lens or specimen Carefully clean the optics and specimen slide vessel Observe again and repeat until view is free of dirt A ghost image of the sample is observed Condenser aperture diap...

Page 44: ...t any stage plate inserts are completely installed and flush with the stage surface Ensure that the sample slide or vessel is completely flat on the stage The optical performance e g flatness of the culture dish bottom is poor Use a culture vessel with good flatness Confirm quality of the culture vessel before seeding cells 10 2 ELECTRICAL Problem Cause Corrective Measure LED bulb does not light P...

Page 45: ...justed Adjust the diopter of one eyepiece so that the focus of the image matches that seen through the other eyepiece Still adapting to viewing through the eyepieces of this microscope It takes time to adjust to a different microscope Try looking around the periphery of the field of view before concentrating on the specimen Looking far away from the microscope e g at an object across the room befo...

Page 46: ...ust free cloth moisten with a neutral cleaner in water and wipe 3 If the microscope is wet by liquid during operation immediately turn off the power supply before cleaning up the liquid 4 Do not disassemble any microscope components This will immediately void any warranty and the performance and function of the microscope may be affected 5 If not filling every position on the nosepiece with an obj...

Page 47: ...assumed for unsatisfactory operating performance due to environmental conditions such as humidity dust corrosive chemicals deposition of oil or other foreign matter spillage or other conditions beyond the control of ACCU SCOPE INC This warranty expressly excludes any liability by ACCU SCOPE INC for consequential loss or damage on any grounds such as but not limited to the non availability to the E...