Euromex OXION Series, User Manual

Page 1: ...page1 16 euromex microscopen bv The Netherlands www euromex com ...

Page 2: ...6 x 50 mm translation mechanical stage and a reversed nosepiece for up to 5 objectives makes the Oxion suitable for advanced laboratory work Ergonomically placed adjustment knobs minimize fatigue during long microscopy sessions The general shape of the stand has been specifically designed to match today s standard The height adjustable Abbe N A 1 25 condenser accepts sliders for economical phase c...

Page 3: ...S100x phase plan objectives The second one allows bright field and phase plan contrast for 10x S40x S100x phase plan objectives and also features a dark field contrast method for magnifications up to 400x Both condensers are delivered with a telescope to adjust the phase contrast rings Fluorescence Today fluorescence microscopy offers a faster and reliable identification of infectious diseases and...

Page 4: ...yeshades 8 Connecting the power cord 8 Operation 9 Setting up the illumination 9 Place the specimen slide 9 Focusing and slide protection 9 Adjusting the focusing tension 9 The interpupillary distance 10 The correct eye point 10 People wearing glasses 10 Adjusting the diopter 10 Abbe condenser 11 The field Köhler diaphragm 11 Adjusting the Aperture Diaphragm 11 Use of the S100x oil immersion objec...

Page 5: ... 14 Using the Dark Field Slider 14 Using the simple polarizing set 15 Using the 0 5x Inch CS C mount camera adapter AE 5057 for trinoculair models only 15 Using the SLR camera adapter AE 5527 for trinoculair models only 15 Maintenance and cleaning 16 Cleaning the optics 16 Maintenance of the stand 16 Accessories 17 Eyepieces and eyeshades 17 Objectives 17 Fluorescence 17 Sliders and phase contrast...

Page 6: ...ves C Set screw for tube P Object clip D Stand arm Q Object x y stage E Stage controls R Condenser with iris diaphragm F Coaxial fine adjustment S Height adjustment condenser not visible G Coaxial coarse adjustment T Köhler field diaphragm filter holder H On Off switch with light adjustment X Slide protection screw I Lamp unit J Eyepieces K Eye cups L Diopter adjustment M Filter slot with dust cov...

Page 7: ...es damp dust or acute shake Please make sure the worktable is flat and horizontal When moving the microscope use both hands to hold the microscope Hold it at the back handle and the front of the base see Fig 1 point 1 Caution Holding the microscope at the table stage focusing knob or head will damage the microscope For safety reasons make sure the power switch is turned off 0 and remove the plug b...

Page 8: ...X to search for specimen and focus and then continue with high magnification objective to observe The microscope head The standard Oxion series configuration is supplied with the head assembled However if your order contains the fluorescence it should be mounted first The dovetail on the bottom side of these parts fits into the slot on the top side of the other parts Placing the eye pieces 1 Remov...

Page 9: ...p 3 Rotating the X and Y axis knob will move the specimen to the center for alignment with the center of the objective Focusing and slide protection 1 Select the objective 4x to the optical path 2 Rotate the position screw to top observe the right eyepiece with right eye Rotate the coarse focusing knob until the image appears 3 Rotate the fine focusing knob for detailed focusing and lock the slide...

Page 10: ... The correct eye point The eye point is the distance from the eyepiece to the user s pupil To obtain the correct eye point move the eyes towards the eyepieces until a sharp image is reached at a full field of view People wearing glasses When the eyeshades bother people wearing glasses the Oxion series eyeshades simply can be folded back to create more space Adjusting the diopter Using a binocular ...

Page 11: ... field of view 6 Open the field diaphragm gradually If the image is in the center all the time and inscribed to the field of view it shows condenser has been centered correctly The field Köhler diaphragm By limiting the diameter of the beam entering the condenser the field diaphragm can prevent other light and strengthen the image contrast When the image is just on the edge of the field of view th...

Page 12: ...le turn the S100x objective a couple of times left right so that the front of the objective moves in the oil and the bubbles will disappear 10 After using the S100x objective turn the table with the fine adjustment knobs downwards until the front lens doesn t touch the oil any longer 11 Always clean the front lens of the S100x objective with a piece of lens paper that is moistened with a drop of X...

Page 13: ...ve is used the slider should be positions to match the 10 phase diaphragm When observing phase contrast please set the aperture diaphragm indicator to PH position Note the phase diaphragms in the sliders are pre centered do not need to be adjusted in operation Mounting and operation of the Euromex Zernike phase contrast condenser set Use of phase contrast with the Oxion microscope The phase contra...

Page 14: ...g phase ring is in place under the condenser Place the centering telescope in the eyepiece tube and focus the phase ring of the objective by means of the adjustable eye lens Now focus the centering ring of the condenser by means of the coarse and fine adjustment knobs At last center the phase ring beneath the condenser disc with the phase contrast adjusting levers until the two rings visible in th...

Page 15: ...ording to the direction of the figure Secure the lock screw tightly 4 Focus on a specimen through binocular using your eyes then observe the image through camera if the image is un sharp rotate the adjustment tube to focus the image Using the SLR camera adapter AE 5527 for trinoculair models only 1 Loosen the lock screw on the trinocular head and remove the dust cover 2 Insert SLR camera adapter t...

Page 16: ...recommends using Euromex cleaning accessories for your Oxion microscope It is not necessary and not recommended to clean the lens surfaces at the inner side of the objectives Sometimes dust can be removed with high pressured air There will never be dust in the objectives if the objectives are not removed from the revolving nosepiece Caution Cleaning cloths containing plastic fibres can damage the ...

Page 17: ...t AE 3144 Infinity Plan Semi Apochromatic 4x objective AE 3152 Infinity Plan Semi Apochromatic S40x objective AE 3146 Infinity Plan Semi Apochromatic 10x objective AE 3156 Infinity Plan Semi Apochromatic S100x objective AE 3148 Infinity Plan Semi Apochromatic 20x objective Fluorescence AE 3240 Fluorescence illuminator including power supply AE 3245 Fluorescence filter block for eGFP FITC Alexa Flu...

Page 18: ... AE 3198 Blue filter 45 mm AE 3200 Yellow filter 45 mm AE 3202 Green filter 45 mm AE 5057 Photo adapter 0 5x magnification for inch C CS mount camera AE 5527 Photo adapter for SLR camera without T2 adapter AE 5040 T2 adapter for Canon EOS SLR camera AE 5025 T2 adapter for Nikon SLR camera AE 5045 3 2x lens for AE 5527 PB 5155 Microscope 76 x 26 mm glass slides edges grinded packed per 50 pieces PB...