iota Sciences isoHub, User Manual

Page 1: ...2 1 0 3 isoHub user manual...

Page 2: ...e reasonable protection against harmful interference in a residential installation This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with...

Page 3: ...Attach controller 10 3 2 6 Insert power cord 11 3 2 7 Attach 60mm dish holder or well plate holder 11 4 Using the isoHub 11 4 1 Powering on 11 4 2 Moving the stage 12 4 3 Running programs 12 4 4 Chamb...

Page 4: ...phase contrast observation 21 7 1 Changing between bright field and phase contrast observation 21 7 2 Adjusting the aperture iris diaphragm 21 7 3 Selecting the objective 22 7 4 Changing between visu...

Page 5: ...Troubleshooting 26 12 Disposal and recycling 29 12 1 Disposal of isoHub 29 12 1 1 EU Waste Electrical and Electronic Equipment WEEE Directive 29 12 1 2 Users outside the European Union 30 13 Technical...

Page 6: ...anner not specified by the manufacturer then the protection provided by the isoHub may be impaired Powering the equipment o Use only with the supplied XP POWER VEC50US24 24VDC 50W power supply and pow...

Page 7: ...formance and damage the isoHub o Ambient temperature 16 35 C 60 95 F o Humidity 40 60 o Altitude less than 2000m o Pollution degree 2 All services shall be performed by iotaSciences authorised personn...

Page 8: ...7 Figure 1 Front view showing key components of the isoHub Figure 2 Back view showing key components of the isoHub...

Page 9: ...60mm dish holder Well plate holder Dust cover Heated centre plate optional 3 2 Installation 3 2 1 Remove isoHub from packaging and place on a flat stable surface When carrying the isoHub to a differen...

Page 10: ...nob a to the extent that it does not fall off Hold the condenser diagonally contact the attaching portion on the top surface b of the condenser to the round dovetail portion on the bottom of the colum...

Page 11: ...ve Attach caps to screw holes a of the revolving nosepiece where no objectives are attached to avoid dust or dirt getting on the lenses After all objectives and caps are attached fit the stage centre...

Page 12: ...c or the well plate holder into position and release points a and b Ensure the 60mm dish holder or well plate holder are flat on the stage 4 Using the isoHub 4 1 Powering on To power on the isoHub en...

Page 13: ...dentify program can be run to review the chambers in a recently plated GRID and mark chambers containing single cells monoclonal chambers Ensure the 60mm dish holder is attached to the isoHub before s...

Page 14: ...chscreen by a blue circle in the above example the isoHub is viewing chamber A01 A larger icon to the left of the GRID shows the state of the chamber that is currently being viewed All programs on the...

Page 15: ...t recent run of the Extract program on the isoCell are distinguished by a small circle in their corner These chambers are the only ones that can be selected in the Prep Redetach program Inactive The c...

Page 16: ...ee the individual sections on each program in section 0 for more details For continuous scanning of chambers press and hold the lower button on the controller How long the isoHub will remain on each c...

Page 17: ...Speed This option will set how fast the isoHub scans through chambers when holding down the button on the joystick to jump between chambers 4 10 3 Centre If the chamber isn t centred in the field of v...

Page 18: ...chambers that are no longer of interest can be marked as inactive and will no longer be included in the Cloning Platform workflow The isoHub will only go to filled clonal chambers to allow users to m...

Page 19: ...n 5 4 2 Suspension cells If the dish doesn t contain any chambers that have had dissociation reagent added to them then the isoHub will move to all filled clonal chambers during the Prep Extract progr...

Page 20: ...sing the controller Turn bed heater on or off Set the time before the isoHub goes to sleep see section 4 11 6 3 Set time The set time program more settings set time is used to update the date and time...

Page 21: ...one isoCell isoHub isn t currently set up to sync data with any isoCells Figure 6 Data syncing status screen accessed by pressing the data syncing status icon on a menu screen The device name display...

Page 22: ...ider within the range of 30deg Select the direction that makes it easy to observe When performing bright field observation Slide the phase contrast slider a and engage the empty hole of the slider in...

Page 23: ...and camera light path Set the light path selection lever to the visual light path for using the eyepiece or camera light path for using a camera Visual light path 100 Camera light path 0 Visual light...

Page 24: ...ghter the brightness becomes 8 Advanced observation 8 1 Adjusting binocular position 8 1 1 Adjusting the interpupillary distance While looking through the eyepieces move the binocular portion a until...

Page 25: ...same manner while looking into the left eyepiece with your left eye rotate the left diopter adjustment ring b to bring the specimen into focus In this case do not touch the coarse fine focusing knobs...

Page 26: ...n this case turn the tension adjustment ring b in the arrow direction to increase the tension of the coarse focusing knob 9 Recording with the camera The observed image can be acquired by attaching th...

Page 27: ...ntering the isoHub may lead to degraded performance or electrical safety risk Use the provided dust cover when isoHub not in use to prevent dust 11 Troubleshooting Problem Cause Remedy The field of vi...

Page 28: ...uorescence observation The fluorescence mirror unit is not properly engaged in the light path Engage the fluorescence mirror unit in the light path correctly Fluorescence observation The shutter for r...

Page 29: ...scence observation The direction of the filter slider for reflected light illumination which is inserted to the fluorescence illuminator is not correct Set the direction of the filter slider for refle...

Page 30: ...ot adjusted appropriately Adjust the tension of the coarse focusing knob to be lighter see section 8 2 The dish data on isoHub doesn t match the dish data on the isoCell The isoCell and isoHub are no...

Page 31: ...se of your isoHub at the end of its useful life please refer to your local waste management organisation for proper disposal practices for used electronic and electrical equipment 13 Technical support...

Page 32: ...ch of statutory duty or otherwise arising under or in connection with the purchase of this instrument for loss of profits loss of business loss of revenue loss of agreements loss of contracts loss of...

Page 33: ...user manuals at any time without notice and the recipient is strongly encouraged to regularly check for updates on the iotaSciences customer portal The isoHub is manufactured by Iota Sciences Ltd Begb...