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Brief Manual

Leica TCS SP2, Brief Manual

Introducing the Leica TCS SP2 - a state-of-the-art imaging system for advanced microscopy. Our Brief Manual provides concise guidance for using the SP2, available for free download at 88.208.23.73:8080. Discover unparalleled image quality and explore the capabilities of this powerful instrument with our user-friendly manual.

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Brief manual how to start and close the Leica sp2 Confocal

(TCS SP2 AOBS system mounted on a DM IRE2)

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Summary of Contents for TCS SP2

Page 1: ...Brief manual how to start and close the Leica sp2 Confocal TCS SP2 AOBS system mounted on a DM IRE2 ...

Page 2: ...the scan head and lasers switches on Press red knob 3 on the cooling is functioning note that outside working hours an additional cooling should be switched on from the box on the corner just outside the room ask Liesbeth Switch the conventional 50 W HBO fluorescence lamp on by pressing once on 20a of EBQ 100 NEVER re ignate the lamp soon after having switched it off In general avoid any unnecessa...

Page 3: ...answering Yes to the question Initialize Stage Remember to perform xy stage initialization only with a 10x objective upright and to press the condenser arm backward if you use the xz galvo sample holder so that does not touch the condenser during the calibration move Click No to the question Initialize Stage if automated xy positioning is not applicable Choose the appropriate objective and immersi...

Page 4: ...out for bright field vision Keep 22 pressed in half pulled out is 50 ocular 50 upper camera port vision fully pulled out is 100 upper camera port vision Ring 23a display 23b can be set in position 1x open connection for bright field UV for ultraviolet scan stand B for phase contrast adjustment with Bertrand lens not relevant in this microscope and scan for non UV scanning Optional but easy is the ...

Page 5: ... laser choose passed through Neutral density filter means no neutral density filter Set PMTs for the desired wavelength ranges by mouse dragging trans active optional reflection is a special case Functions of the black knobs can be adjusted by right clicking on the lower bar menu choose configuration for menu choose sensitivity to adjust the coarsness of the knob e g Smart offset smart gain Use th...

Page 6: ...lly with the smart function knobs on the table panel or automatically with A Gain There is an extensive Help menu within the Leica Confocal software as well as a print of most help functions and tutorials in the confocal microscope room Acquired images are stored temporarily on a temp directory can be retrieved if the program crashes but not if the computer crashes therefore it is advisable to reg...

Page 7: ...since we had to remove F secure to save space on the 10G C drive USERS SHOULD REMOVE THEIR OWN DATA FROM THE HARD DISK WITHIN ONE WEEK to prevent congestion of the system Shut down Windows on the computer When ready switch 3 out Switch 2 out Switch 1 out D Good working practice Always reserve time through bookings science ru nl Do not hesitate to contact Liesbeth for assistance instructions if you...

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