NanoEnTek ADAM-MC, User Manual

Page 1: ...USER MANUAL NESMU AMC 001E V 6 1 ADAM MC A New Standard of Automated Cell Counter E x p l o r e t h e C e l l u l a r U n i v e r s e...

Page 2: ...9 NanoEntek America Inc 220 Bear Hill Road Suite 102 Waltham MA 02451 USA Tel 1 781 472 2558 Fax 1 781 790 5649 The information in this manual is described as correctly as possible and is applicable t...

Page 3: ...ror message during booting 16 Menu setting 17 System information 17 Chip selection 18 Counting method 19 General operation 20 25 Introduction 20 Preparing cell 20 Counting cell 20 Operating ADAM MC 21...

Page 4: ...1 x 12 5ml Min 200 test kit Max 400 test kit Only total cell count Accuchip4x Kit Cat No AD4K 200 200pcs 4 channel 2 x 12 5ml 1 x 12 5ml Min 400 test kit Max 800 test kit Only total cell count AccuSt...

Page 5: ...ing terminal of the instrument and that of the wall outlet are properly connected The power cord should be connected to a grounded 3 conductor power outlet 7 To avoid potential shock hazard make sure...

Page 6: ...easures must be used in dealing with biologically hazardous materials such as carcinogenic reagents Disposal of your old appliance 1 When this crossed out wheeled bin symbol is attached to a product i...

Page 7: ...nless this manual has been consulted and is understood This manual is available in English only Failure to heed this warning may result in injury to service provider operator from electric shock mecha...

Page 8: ...tal cells T and non viable cells N 5 104 to 4 106 cells mL 45 sec 2min test 20 L test AccuChip2x 13 L test AccuChip4X 8 5 L test AccuChip2X 3 L test AccuChip4X 1 5 m 250 VAC 3 A F3 15AL250V USB Type 1...

Page 9: ...he ADAM MC is shown below Key Pad Control Button Door LCD Control buttons Eject Ejects the chip holder from the ADAM MC Start Performs all procedures of automatic counting Lock Protects the alignment...

Page 10: ...Fan Key Pad Port RS232C Port USB Port Video Port Fan Cooling fan Power switch Main power on off switch Power plug ADAM MC power cord connection plug USB port Connect to computer with USB Cable RS232C...

Page 11: ...ommon variation of counts normally associated with this method To address these problems NanoEntek has developed the ADAM MC which is based on a fluorescent microscopy technique for counting cells The...

Page 12: ...scent dye PI and lysis solution AccuStain solution N for the non viable cell counting is composed of the fluorescent dye and PBS In order to measure the total concentration of cells the plasma membran...

Page 13: ...mperature 10 C warm up the ADAM MC for 10 min 2 Not exposed to direct sun light 3 Not subject to direct or continuous vibration 4 Not subject to intense magnetic or electromagnetic fields 5 Relative h...

Page 14: ...rface 3 Plug to power cord into the electrical outlet Be sure to use only the power cord supplied with your instrument Powering the instrument with an unapproved power cord may damaged the instrument...

Page 15: ...tting started Start up screen 1 System booting 2 System will go through self diagnostic tests If you get an error message please contact your local distributor or sales nanoentek com 3 The home screen...

Page 16: ...k com 1 It appears when booting not working properly 2 Turn off main power and restart device 3 If this message still appears after restart contact your local distributor or sales nanoentek com 1 It a...

Page 17: ...rom the screen for inputting sample numbers 2 You can select the number from the menu 1 Check the device versions and date which have been installed in the device 2 Select number 4 key from the menu a...

Page 18: ...ll return to the menu screen auto matically 1 Press 1 for chip selection You can select the number from the menu 2 User can select the 2 kinds of chip type 1 Two channel chip AccuChip2X 2 Four channel...

Page 19: ...to the menu screen auto matically 1 Press 2 for counting method You can change the capture frame mode 2 You can select the 60 or 30 frames in 2CH mode and 22 or 11 frames in 4CH mode for counting as w...

Page 20: ...unt using the ADAM MC AccuChip 2x AccuChip 4x Total Cell 1 Add 50 L of your sample to 50 L supplied AccuStain solution T 2 Mix gently by pipetting up and down 3 Load 20 L sample mixture to the AccuChi...

Page 21: ...t takes approximately 90 seconds to count sample 5 After calculating the cell number the chip will be ejected automatically Then chip can be removed 6 The calculated cell number per 1ml will be displa...

Page 22: ...g is running After pressing the Eject button Shows the Chip Holder is parked After pressing the Lock button Shows the Chip Holder is inserted After pressing the Eject button with AccuChip Error code g...

Page 23: ...l N1 5 50E5 N2 5 50E5 Viability 50 75 1 10E6 1 10 X 106 cells mL The viability will be automatically calculated by the ADAM MC software after each measurement of the total cells and the non viable cel...

Page 24: ...of total counting frame Frame with error is a frame that contains cells whose diameter is larger than 30 m Cellsaremorethan4X106 cells mL Over detection range will be shown on report program Cellsarem...

Page 25: ...ce 2 ADAM MC has no replacement of consumable materials 3 Clean the exposed outer surface of ADAM MC using a soft cloth and isopropyl alcohol or deionizers water CAUTION Dispose of wipes in an appropr...

Page 26: ...ages into desktop or laptop hard drive The data list window consists of the sample number chip date time total nonviable viable viability counting result in CAUTION Before running the program check th...

Page 27: ...o the computer Then open the file Setup_ADAM_v1 x x x exe Report program can be installed in Windows 2000 XP or higher version 2 The start up dialogue of the software as shown like left image will app...

Page 28: ...the InstallationoftheSoftware Initial installation folder is C Program Files Digital Bio ADAM 7 Report program will be installed automatically 8 Click Ok to finish the installation 9 If the installati...

Page 29: ...s exporting data and all function of Report Program are handled by using these buttons see p 29 for more detailed information of each button All saved data in ADAM MC will be loaded and shown in data...

Page 30: ...he data list to Excel format and saves it Turn on or off automatic image save option Default image save folder is C Program Files Digital Bio ADAM Images Images will be saved until your hard drive has...

Page 31: ...utomatically saved to ADAM MC memory When memory of ADAM MC is full new counting result will replace old data These data can be exported as Excel Sheet xls and stored in personal computer or can be er...

Page 32: ...istribution of cell size The size of cell in graph is not real size of cell It is the size of pixels in fluorescence image captured by ADAM MC And the size information is only to judge if there is a l...

Page 33: ...ADAM MC Automated Cell Counter 2008 NanoEntek Inc 33 Sample image of error message Software installation...

Page 34: ...source 3 Replace 1 Adjust the number of cells between 5X104 4X106 cells mL refer to page 21 2 Check the expired date 3 Try again after vortexing the cells 1 Check program setup 2 Check all connections...

Page 35: ...Entek or an authorized agent Damage caused by substituting alternative parts Use of fittings or spare parts supplied by anyone other than NanoEntek Damage caused by accident or misuse Damage caused by...

Page 36: ...Kit AccuStain Contents 7 LCD Monitor 200 pcs AccuChip2X 12 5 ml AccuStain solution T 12 5 ml AccuStain solution N 200 pcs AccuChip4X 12 5 ml AccuStain solution T 12 5 ml AccuStain solution N 12 5 ml A...

Page 37: ...ADAM MC Automated Cell Counter 2008 NanoEntek Inc 37...

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Page 39: ...ADAM MC Automated Cell Counter 2008 NanoEntek Inc 39...

Page 40: ...Developed and Manufactured by NanoEntek Inc NanoEntek Inc 851 14 Seohae ro Paltan myeon Hwaseong si Gyeonggi do 18531 Korea Tel 82 2 6220 7940 Fax 82 2 6220 7999 NanoEntek America Inc 220 Bear Hill R...