Nikon LABOPHOT2-POL, Instructions Manual

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Page 2: ...Input voltage to the mlcro scope corresponds to your line voltage BLight Source Use a 6V 30W halogen lamp Do not use lamps other than the one specified on p 41 Electrical Specifications If a lamp of more than the suggested wattage IS used the light adjusting circuit may be damaged When the Lamp Is On Take care not to touch the lamp housing or bring Inflammable substances such as gasoline thinner a...

Page 3: ...the objectives of immersion oil use only xylene Do not use xylene for cleaning the surface of the entrance lens of the eyepiece tube or the prism surface of the Ultra Wide Eyepiece Tube UW Observe sufficient caution In handling alcohol and xylene they are inflammable and the ON OFF of the power source sWitch fjCleaning the Painted Surfaces Avoid the use of any organic solvent for example thinner e...

Page 4: ...tage clamp screw Condenser centering screw Condenser aperture diaphragm control ring Field diaphragm control ring Power switch Brightness adjuster Binocular eyepiece tube Eyepiece tube clamp screw Bertrand lens centering screw Analyzer knob Intermediate tube clamp screw Revolving nosepiece clamp screw Coarse focus torque adjustment ring Fine focus scale Lamp hOUSing Arm rest Fig 1 1 ...

Page 5: ...BLY II ASSEMBLY To assemble the microscope follow the procedures given from CD to For details read p 5 to p 10 a 6 t CV Ptionall Fig 2 Tools hexagon wrench accessory plus screwdriver minus screwdriver coin etc ...

Page 6: ... Coarse focus knob Fine focus knob Power cord Condenser focus knob Condenser clamp screw ND filter knob I NOMENCLATURE Eyepiece Diopter ring Bertrand lens turret Bertrand lens focus ring 1 4 tint plate Vernier Stage rotation clamp screw Condenser top lens swing out knob Optional Fig 1 2 2 ...

Page 7: ...our fingers If the bulb surface IS contaminated with finger marks or dirt wipe them off with lens tissue Be sure to remove the lamp cover after mounting the lamp Remove the lamp housing cover pressing the cover detaching buttons Fig 2 2 elnsert the lamp Into the socket pin holes until it reaches the limit Fig 2 3 Reattach the cover Cautions when dismounting Turn oft the power switch Assure that It...

Page 8: ...ing the coarse focus knob Loosen the stage clamp screw sufficiently Fig 2 4 Fit the stage to the circular dovetail of the substage 00 Fasten the stage with the stage clamp screw Fig 2 5 Fig 2 5 Fig 2 6 Specimen Clip Insert the two speciment clips into the holes on the stage surface Fig 2 6 Condenser Raise the substage by turning the coarse focus knob Lower the condenser carrier to its limit by tur...

Page 9: ...e Mount the intermediate tube onto the microscope arm fitting the notch of the circular devetail of the intermediate tube with the end of the clamp screw Tighten the clamp screw holding the mid stem of the hexgon wrench Do not over tighten the screw holding the L shaped holder of the wrench Fig 2 9 1 4A Tint Plate Remove the screw from the side of the 1 4 A plate Insert the plate Into the compensa...

Page 10: ... be malfunctioned QJ Eyepiece e Use the same magnification eyepieces for both the right and left eyes elnsert the eyepieces into the sleeves of the binocular eyepiece tube by engaging the three grooves of the eyepiece with the three protrusions of the sleeve Fig 2 12 e When using eyeguard rubbers put them onto the eyepieces Fig 2 13 Fig 2 12 Rotation clamp ring for the eyepiece Fig 2 13 The rotati...

Page 11: ... O 8 diameter protrusion Pawls Rotation clamp ring for the eyepiece Grooves Filter on the Filter Receptacle Binocular eyepiece tube sleeve Fig 2 15 Place the 45mm filter on the filter receptacle of the field lens part Fig 2 16 Fig 2 16 Fig 2 17 ND Filter Cassette optional Push down the ND filter cassette so the two protrusions of the cassette bottom fit with the two mounting grooves on the rim of ...

Page 12: ...ase of using the extension cord use only the power supply cord including PE wire For220 240Varea e Use only the 3 pole power supply cord type H05VV F or H05VVH2 F which must be approved according to DIN VDE 0625 The plug and the outlet are to be approved according to DIN VDE 0620 and DIN VDE 0625 respectively e Class I equipment should be connected to PE protective earth terminal eln case of using...

Page 13: ...ND filter cassette if used Fig 3 2 Refer to II ASSEMBLY p 9 3 Turn on the power switch rn to light the lamp Move the brightness adjuster and align it with the 3rd scale line from the right Fig 3 3 4 Pull the analyzer knob of the intermediate tube rn to remove the analyzer from the optical path Turn the Bertrand lens turret to the position 0 to remove the Bertrand lens from the optical path Fig 3 4...

Page 14: ... Fig 3 5 6 Revolve the revolving nosepiece to swing in the 10 x objective to the optical path Assure that the nosepiece properly settles in position Fig 3 6 7 Place the specimen glass slide on the stage with its cover glass facing up and fasten it with the two specimen clips Fig 3 7 8 Raise the condenser uppermost using the condenser focus knob Fig 3 8 12 ...

Page 15: ...men into the optical path Fig 3 1 0 Object portion IS just above the condenser lens 11 When the trinocular eyepiece tube is used change the optical path of the eyepiece tube to enter 100 of the light into the binocular part Fig 3 11 See p 24 Operations in Detail 11 12 Focus on the specimen looking in the eyepiece by manipulating the coarse fine focus knob Fig 3 12 ...

Page 16: ...Ill MICROSCOP 13 Adjust the diopter Fig 3 13 See p 25 Operations in Detail 1 3 14 Adjust the interpupillary distance Fig 3 1 4 See p 25 Operations in Detail 1 2 15 Center the obj ective Fig 3 15 See p 20 Operations in Detail 5 14 ...

Page 17: ...e or coarse focus knob See Note 2 When using an oil immersion objective apply oil to the space between the top of the objective and the specimen Take care not to produce bubbles in the oil See p 26 Operations In Detail 14 19 When the ND filter cassette is used Adjust the brightness by sliding the filter knob See p 27 Operations In Detail l 5 When the N D filter cassette is not used adjust the brig...

Page 18: ...entering screws if part of the viewfield is dark If this doesn t help check the following items elnsertion removal of the ND filter eTurning the revolving nosepiece click stop position e Position of the condenser eViewfield and aperture diaphragms fully open e Change over of the optical path of the eyepiece tube e Mounting the lamp e Mounting the condenser e Placing the filter on the filter recept...

Page 19: ...o the optical path Observing the exit pupil of objective inside rotate the dia polarizer so as to form a dark cross image on the exit pupil as shown in Fig 5 eThe analyzer can be rotated by loosening the analyzer clamp screw rn and rotating the rotation ring The rotation angle can be read in the range between O to 180 with the unit of 0 1 by the vernier The analyzer can be removed from the optical...

Page 20: ...ge and the two centering screws Fig 7 Centering procedure for the Bertrand lens is the same as that for the condenser except that the condenser aperture diaphragm image is used in place of the field diaphragm image In condenser contering Refer to Item 8 Note I Another Bertrand lens IS built in the optional tnnocular eyepiece tube Turn the Bertrand lens nng to the position B and the Bertrand lens w...

Page 21: ...When measuring the retardation or observing the interference color swing out the top lens or stop down the condenser aperture diaphragm so as to make the illumination light flux as parallel as possible to the optical axis Fig 9 4 1 4 t Tint Plate eThe 1 4 tint plate has an empty hole at the center By pushing it into the compensator slot the sensitive tint plate 530nm is brought into the optical pa...

Page 22: ...tools in the centering screws on the nosepiece Fig 10 J Rotate the stage about 180 0 and the target is displaced from the center of the cross lines Move the objective using the centering tools so that the center of the cross lines moves one half the displacement of the target Fig 11 e Repeat the above procedure two or three times e Carry out centeri ng for each objective Fig 10 6 Stage Rotation Ci...

Page 23: ...3 e One rotation of the fine focus knob moves the stage 0 1 mm and the graduation on the fine focus knob is 1 micron One rotation of the coarse focus knob moves the stage 12mm The range of coarse and fine motion is 2mm up and 28mm down from the standard position Never turn the right or left knob while holding the other It may cause problems Do not turn the coarse and fine focus knobs further than ...

Page 24: ...ce Fig 14 rn Image of field diaphragm Fig 14 2 If the image decenters from the viewfield of the eyepiece bring it roughly to the center of the viewfield by means of the condenser centering screws Figs 14 Z and 15 Q Change to the 40 x objective Focus on the specimen by turning the fine focus knob and form an image of the field diaphragm on the specimen surface by manipulating the condenser focus kn...

Page 25: ...ipulate the diaphragm control ring referring to the condenser s N A scale or observing the diaphragm Image VISible on the exit pupil inside the objective The eXit pupil can be seen by removing the eyepiece from the eyepiece tube or putting the Bertrand lens into the optical path Stopping down the aperture diaphragm too far will lower the resolVing power Therefore it IS recommended not to stop down...

Page 26: ...t the diameter of the illuminated area is slightly larger than the diagonal of the film format Note eThickness of the glass slide must be 1 7mm or less otherwise the field diaphragm image may not be focused on the specimen surface eThis diaphragm does not work as the field diaphragm when the condenser top lens IS swung out of the optical path In this case fully open the diaphragm as the numerical ...

Page 27: ...pter Adjustment Eyepiece Before adjusting the diopter implement MICROSCOPY procedures 1 to 12 and focus on the specimen with the lOx objective Make diopter adjustments for both the right and left eyepieces CD Turn the diopter compensation rings on each eyepiece until the end surface of the ring coincides with the engraved line This is the position of 0 dioptic compensation Fig 19 1 Swing in the 40...

Page 28: ...ount necessary the quantity that fills the gap between the front of the objective and the specimen to avoid a flow of excessive oil that will adhere to the stage To see if air bubbles are present in the immersion oil which deteriorate the image quality pullout the eyepiece from the eyepiece tube Fully open the viewfield diaphragm and the aperture diaphragm to examine the objective exit pupil brigh...

Page 29: ...eniently used for brightness adjustment In general microscopy and photomicrography Illumination brightness can be reduced to 1 2 1 128 by the combined use of these filters Securely change the filter knob when inserting or removing the filters Any filters of 45mm in outer diameter and 3mm or less in thickness ca n be mou nted to the ND fi Iter cassette Filters on Filter Receptacle Table 2 Name Purp...

Page 30: ...the ND filter cassette to protect the filters from dust Fig 22 1 When the ND filter cass ette is removed place the transparent cover on the field lens Fig 22 2 However remove the cover during observation or photomicrographing with the viewfield diaphragm stopped down Fig 22 1 Fig 22 2 e Other filters 33mm Lemon skin filter When the optional achromat condenser is used insert the 33m m lemon skin fi...

Page 31: ...e direct image produced by the objective can be utilized for observation by a TV camera etc With the objectives marked 160 0 1 T use a coverglass of O 17mm in thickness The indication 160 on the objective means that no matter whether a coverglass is used CJr not no decrease of image definition or of contrast will result When the eyepiece with cross lines and scale is Inserted into the eyepiece sle...

Page 32: ...xtinction position to the diagonal position Insert the 1 4 1 tint plate into the optical path and confirm that the interference color of the part of the specimen to be measured changes toward the lower order side If the color changes toward the higher order side rotate the stage another 90 3 Measurement Place the GI F filter on the filter receptacle and replace the 1 4 1 tint plate with the compen...

Page 33: ...terference color of the part of the specimen to be measured changes toward the lower order side If the color changes toward the higher order side rotate the stage another 90 3 Measurement Slide the quartz wedge along the slot and the interference color changes Stop sliding the quartz wedge where the dark stripe covers the part of the specimen to be measured In this position find the part with no o...

Page 34: ...t flux that covers the central part of the orthoscopic view field to the extent of about 1 20 Fig 26 4 Attachable Mechanical Stage Mount the attachable mechanical stage on the circular graduated stage Inserting the two positioning pins on the bottom of the attachable stage Into the two pin holes on the graduated stage surface Tighten the clamp screw using a screwdriver or coin Fig 27 The attachabl...

Page 35: ...ator Retighten the clamp screw Connect the lamp cord to the transformer Remove the accessory ND32 filter slider from the illuminator Insert the polarizer slider into the illuminator until the slider clicks twice Place the filters j Mount the intermediate tube onto the illuminator fitting the notch of the circular dovetail with the end of the clamp screw Fasten the clamp screw Referring to p 8 moun...

Page 36: ...pulate the lamp centering screws to center the filament image on the exit pupil 2 Orientation of polarizer CD Roughly focus on the ND filter on the stage using the 40 x objective 2 Set the analyzer scale to 0 Q Bring the Bertrand lens into the optical path Looking into the exit pupil of the objective rotate the polarizer rotation ring to form the dark cross Image on the exit pupil Fig 29 Note I Ta...

Page 37: ...t the Condenser not centered Centering p 22 periphery Condenser not mounted correctly Mount correctly p 6 Uneven NO filter not fully changed over Changeover to the limit brightness of viewfield Field diaphragm closed too much Open properly p 24 No appearance Improper combination of objective Use proper combination of viewfield and condenser Dirt or dust on the lens field lens condenser objective e...

Page 38: ...tive for observing Use normal objective for specimen without coverglass used observing specimen with to observe specimen with coverglas s coverglass Poor image Normal objective for observing obtained specimen with coverglass used to Use NCG objective Contrast is too observe specimen without strong or too coverglass weak No immerSion oil used on the front Details are not of immerSion system objecti...

Page 39: ...ng p 22 centered Stage tilted Attach correctly p 6 NCB 11 filter not used Use NCB11 filter p 11 Image tinged yellow Lamp power source voltage too Adjust the lamp voltage low by manipulating the Lamp power source voltage too brig htness adJuster Use Image too bright high the optional NO filter cassette If the constant Lamp power source voltage too color temperature is to low be deSIred p 11 27 Aper...

Page 40: ...en Use coverglass of when changed over Coverglass too thick specified thickness from low power 017mm Eyepiece diopter not adjusted Diopter adjustment p25 Insufficient parfocality of Eyepiece diopter not adjusted Diopter adjustment objective when p25 changed over Movement of image not smooth when moving the Attachable stage not tightly fastened specimen Fasten it tightly p32 When optional to stage ...

Page 41: ...ons of right and left Use same eyepieces eyepieces differ Interpupillary distance not adJusted Adjust interpupillary distance p 25 Eyepiece diopter not adJusted Diopter adjustment p 25 Experiencing eye Adjust the lamp voltage fatigue by man Ipulatlng the brightness adjuster Use Inadequate Illumination the optional NO filter cassette and adjust the brightness with l combination of NO filters p 27 3...

Page 42: ...Bulb blown Replacement p 5 Lamp not correctly mounted Attach securely p 5 Bulb about to blow Replacement p 5 Flickering and Connector not secure Connect power source unstable cord securely p l0 illumination Bulb insuffiCiently Inserted Into the Positive connection socket p 5 Bulb immediately blown Use 6V 30W halogen Bulb used not the one specified lamp Insufficient illumination 40 ...

Page 43: ...V DC 30W Lamp type Nikon 6V 30W Halogen lamp 3 Protection Class Class I 4 Coforming Standards This product conforms to DIN VDE0411 and IEC1 01 O 5 Operating Environmental Conditions Room temperature 40 C max Relative humidity 85 max REFERENCE This manual Instructs only how to manipulate the LABOPHOT2 POL microscope For the practical explanation on polarizing microscopy refer to the following speci...

Page 44: ...ht to make such alterations in design as may be considered necessary in the light of experience For this reason particulars and illustration in this handbook may not conform in every detail to models in current production 42 ...