Nikon OPTIPHOT-POL, Instructions Manual

Page 1: ...Nikon Polarizing Microscope OPTIPHOT POL INSTRUCTIONS NIPPON KOGAKU K K ...

Page 2: ...irectly oIn lighting the lamp Take care not to touch the lamp housing being lighted and don t bring inflammable substances such as gasoline thinner and alcohol near to the lamp housing as some parts of the lamp housing may take a high temperature while the lamp is being light ed oExchanging the lamp bulb and fuse Before replacing the lamp bulb or fuse turn 0 FF the power switch and disconnect the ...

Page 3: ...eaning the painted surfaces Avoid the use of any organic solvent for example thinner ether alcohol xylene etc for cleaning the painted surfaces and plastic parts of the instrument oCleaning the lenses To clean the lens surfaces remove dust using a soft brush or gauze Only for removing finger marks or grease should soft cotton cloth lens tissue or gauze lightly moistened with absolute alcohol ethan...

Page 4: ...ntation plate Field lens Brightness control dial including power switch Microflex clamp screw Trinocular eyepiece tube TP Optical path change over knob Eyepiece tube clamp screw Analyzer knob I Intermediate tube clamp screw 111 1 Substage clamp screw Socket sleeve Lamp vertical centering ring Lamp lateral centering screw Lamp input plug Filter receptacle Filter frame ND16 GIFI NCB10 ND2 Dust cap L...

Page 5: ...er focus knob Coarse focus knob Fine focus knob Lamp housing Lamp housing clamp screw Arm rest Compensator slot 1 4 A tint plate Nosepiece centering screw Specimen clip Condenser aperture diaphragm control ring Dia polarizer Condenser clamp screw Brightness indicator Field diaphragm control ring X POL stand Fig 2 ...

Page 6: ...iently Releasing the nosepiece clamp screw on the left side of the microscope arm insert the nose piece Making sure of positive fitting of the pin on the micro scope arm into the nosepiece groove refasten the clamp screw 6CF Achromat P objective Strain free Mount the objectives on the nose piece in such positions that their magnifying power increases as the nosepiece is revolved clockwise lowest p...

Page 7: ...e col lector lens and fas ten the clamp screw on the left side of the housing Filter Place the filter into the filter frame After centering the lamp put the filter with the frame into the filter receptacle No frame is provided for the diffuser Power input plug Connect the plug to the re ceptacle on the fi Iter recep tacle s right side Insert the lamp bulb with its pins into the accepting holes in ...

Page 8: ...g Lamp centering tool 1 Centering the Lamp ND filter 1 Connect the power source cord to the socket 2 Turn the brightness control dial to switch ON and adjust the voltage to 6 on the indicator 3 Place the specimen on the stage and focus on the specimen using lOx objective In this case open the condenser aperture and field diaphragms to the largest extent 4 Roughly center the condenser lens using lO...

Page 9: ...sert the centering tools in the centering screws on the nosepiece F ig 14 2 Interpupillary DistanceAdjustment I Place a specimen on the stage and focus on the specimen As shown in Fig 9 adjust the interpupillary distance so that both the right and left view fields become one Fig 9 Fig 10 3 Diopter Adjustment Rotate the diopter ring on the eyepiece CFW lOx CM until the cross lines are seen clear Fi...

Page 10: ...arry out centering for each objective 6 Centering the Condenser Lens 1 Close the field diaphragm in the micro scope base to its smallest size by means of the field diaphragm control ring Rotate the condenser focus knob to move the condenser vertically so that a sharp image of the field diaphragm is formed on the specimen surface 2 Bring the field diaphragm image to the center of the field of view ...

Page 11: ...procedure for the objective Refer to P 9 8 Carry out the centering procedure for the con denser Refer to P 10 9 Bring the analyzer into the optical path 10 Swing in the objective to be used and refocus on specimen 11 Brightness is adjusted by selecting ND filters or by changing the lamp voltage to 6 12 Table 1 Orthoscopic Conoscopic microscopy IN higher IN 4X or OUT er trand lens OUT IN Circumscri...

Page 12: ... fully open the condenser aperture diaphragm 2 Conoscopic microscopy In conoscopic microscopy the condenser aperture diaphragm works as a field dia phragm on the conoscopic image surface Stop down the diaphragm to such an extent that it circumscribes the circumfer ence of the field of view of the conoscopic image exit pupil of objective to shut out the stray light 3 Field diaphragm F diaphragm The...

Page 13: ...trast will result 6 Eyepieces To take full advantage of the CF eyepieces use them in combination with the CF objectives By inserting the eyepiece with cross lines and graduation CFW 10XCM into the eyepiece sleeve fitting the protractor pin into the right hand side groove of the sleeve the O direction of the analyzer and dia polarizer are al igned with the cross lines direction If the protractor pi...

Page 14: ...t the sensitive tint plate 530nm is brought into the optical path and by pu IIing it out the 1 4 A p late is brought into the optical path 10 Dia polarizer and analyzer When the both are set at read ing on the protractor scale position of the polariza tion plane coincides with the orientation plate which shows that the indication P of the X direction is for dia polarizer and the A of the V directi...

Page 15: ...g 25 The analyzer rotates 1800 via the rotation ring the left hand side clamp being releas ed The rotation angle readable with accuracy of 0 10 via the vernier The analyzer can be taken out of the optical path by pulling out by the analyzer knob Fig 26 Substage clamp screw rtY Fig 27 12 Lowering the substage Releasing the clamp screw using a screw driver as shown in Fig 27 permits lower ing the su...

Page 16: ...lter available from the film manufac tu rer be used 3 Shutter Speed Desirable shutter speeds for least vibration are 1 4 1 15 sec Adjustment of the image bright ness for color photomicrography should be made by means of the NO filters 4 Manipulation of Field and Aperture Diaphragm In photomicrography the adjustment of the field diaphragm is important for the purpose of limiting extraneous light wh...

Page 17: ... frame Intermediate frame For 35mm film For 4 x5 Polaroid film For 3 X 4X Polaroid film For 6X9 roll film Double cross line Finder mask When the mask eyepiece is used select one out of masks in the view field suitable to the film size relative to CF PL Projection lens in use in reference with Fig 32 and Table 4 Fig 31 2 Make focusing according to the magnifica tion of objective to be used Using 40...

Page 18: ...care to the relation between the orientation of the polarizer analyzer and the position of the Microflex When using the 2 X objective it is recom mended to remove the swing out achromat condenser For photomicrography when focusing with the binocular observation tube use the CF eyepiece CF Photo eyepiece and CF Photo Mask eyepiece with the magnifi cation and other indications engraved in yellow or ...

Page 19: ...5 to bring it to the dia gonal position from the extinction position and confirm that the interference color of the specimen part for measurement changes toward the lower order side by inserting the quartz wedge into the optical path If the color changes toward the higher order side rotate the stage further by 900 When the fi Iter GIF is used A 546nm 3 Measurement Inserting the filter GIF into the...

Page 20: ...piece tube and the inter mediate tube P from the X POL stand 2 Mount the universal epi illuminator on the microscope arm positioning the illumi nator nearly parallel to the arm Clamp the screw QJ After releasing sufficiently the clamp screw on the lamp housing to which the lamp bulb 12V 50W Halogen lamp and socket is attached insert the lamp housing into the universal epi illuminator and clamp the...

Page 21: ...ies Strain free 210 45 Dark cross image 2 Preparation 1 Centering the lamp CD Make certain that the optical path change over knob is pushed to the limit Turn ON the power switch on the trans former set the voltage to 6V Q If the L900C filter is in the optical path remove this Fully open the aperture diaphragm 5 Place the ND filter on the stage and focus on it using objective 10 X Remove the eyepie...

Page 22: ...se Refer to P 11 ectly Correct positioning Refer to P 8 ctly Correct attaching Referto P 6 Bertrand lens in the optical path Flip out Refer to P 13 19 Swing out Refer to P 19 e AP Top lens of condenser incorrectly Swing in to the limit 1 4 A tint plate compensator or Correct setting ositioned or dust in Dirt or dust on the lens Cleaning Condenser objective eyepiece field lens Dirt or dust on the s...

Page 23: ...ick stop Revolve it to click stop position sed position Centering nosepiece not clamped Clamp tightly tered Correct centering Refer to P 10 ntered Correct centering Refer to P 8 be not Changing over to the limit Refer to P 9 NCB 10 filter not used Use NCB 10 fi Iter Too low power somce voltage Raise the voltage over 6 on the indicator ND fi Iter not used Use ND filter 2 Manipulation Failures Cause...

Page 24: ...o electricity obtained Connect the cord to socket No lamp bulb attached Attaching Lamp bulb blown Replacement Fuse blown Replacement Input voltage not adjusted to Turn the change over switch on house current voltage the microscope bottom House current voltage fluctuates Use transformer or the like for adequate voltage not made Make adjustment Refer to P 7 rong glare Lowest voltage adjustment not m...

Page 25: ... Correct positioning Refer to P 10 ed Use 12V 50W specified Halogen bulb jective Cleaning Too low voltage Raise the voltage Not specified fuse used Use 1A 250V or O 75A 250V Lamp bulb going to be blown Replacement Connector not connected securely Secure connection Fuse holder not firmly fastened Firm fastening Irregular change of house current Use stabilizer Lamp bulb insufficiently inserted Posit...

Page 26: ...ractical explanation on polarizing microscopy refer to the following special works AN INTRODUCTION TO THE METHODS OF OPTICAL CRYSTALLOGRAPHY F Donald Bloss Holt Rinehart and Winston ORE MICROSCOPY Eugene N Cameron John Wiley Sons Inc THE POLARIZING MICROSCOPE F A Hallimond Vickers Instruments ...

Page 27: ...H III PS or 7027 use 100 120V 1A 250V 220 240V 0 75A 250V We reserve the right to make such alterations in design as we may consider necessary in the light of experience For this reason particulars and illustrations in this handbook may not conform in every detail to models in current production ...

Page 28: ... Nikon NIPPON KOGAKU K K Fuji Bldg 2 3 3 chome Marunouchi Chiyoda ku Tokyo 100 Japan ft03 214 5311 Telex J22601 NIKON Printed in 1apan 86 4 e H E 5r ...