Olympus BX-FLA, Instructions Manual

Page 1: ...ety and obtain optimum performance and to familiarize yourself fully with the use of this attachment we recommend that you study this manual thoroughly before operating the microscope system Retain th...

Page 2: ...rds are properly connected 4 The ultraviolet rays emitted by the burner are harmful Be sure to use a UV protective shield with the unit See page 9 5 Do not open the lamp housing while the burner is tu...

Page 3: ...n adjuster changer rsal reflected light fluorescence vertical illuminator U URA Conoscope attachment w Microscope frame BX40 or BX50 l U OPA Orthoscope attachment Multi viewing attachment U DA Drawing...

Page 4: ...BX FLA...

Page 5: ...ttance and reflect ance it is necessary to use an appropirate combination of exciter and barner filters in conjunction with the dichroic mirror This is necessary to achieve a good contrast image throu...

Page 6: ...Universal reflected light fluorescence vertical illuminator I IMercury lamp housing Power supply unit BX series microscope I I I Universal condenser 0 2...

Page 7: ...Light excluding shutter Filter slider Another is on opposite side Slider insertion slot 1 B Cube Removable index mark Combine with dichroic mirrors barrier filters and exciter filters as appropriate f...

Page 8: ...C Fluorescent Light Source l Mercury Burner Lamp Housing Burner centering knob Burner Socket l Power Supply Unit Burner ON LED Main switch AC receptacle output connector 0 4...

Page 9: ...assemble the parts in the sequence indicated by the numbers in the illustration below Eyepiece Observation tube nrversal reflected orescence vertic UV protective shield Light excluding slide Microsco...

Page 10: ...ponding turret position Fig 2 Ir Use a sharp object such as the tip of a ballpoint pen or mechanical pencil to lift the cube s magnetic index sticker Ir Move the cube s magnetic index sticker WU NU et...

Page 11: ...sing are removed Mounting the Collector Lens 1 Align the collector lens positioning groove with the pin inside the lamp housing pull up the collector lens focusing knob 0 and slide the collector lens...

Page 12: ...urner when it has been used for 200 0 hours The burner may crack if used beyond the specified life time 2 Before replacing the burner wait at least 10 minutes after turning the burner off Before remov...

Page 13: ...to the AC receptacle on the power supply unit then plug the other end of the cord into an AC outlet A Connect the power cord correctly and ensure that the ground terminal of the power supply and that...

Page 14: ...s l Barrier filter 0 1 25 O mm dia 6 mm max thickness l Exciter filter 0 1 0 1 l Dichroic mirrors 26 0 3mm X 38 0 3mm 1 0 05 mm thickness Barrier filter commercially available Dichroic mirror commerci...

Page 15: ...econds Ir To avoid shortening the life of the burner do not turn the burner off within 15 minutes of ignition After turning the burner off it cannot be re ignited until the mercury vapor cools and con...

Page 16: ...ight path 5 Pull out the aperture iris diaphragm knob to bring the shadow of the aperture iris diaphragm into the centering screen BH2 SGRF 6 Adjust the two aperture iris diaphragm centering knobs to...

Page 17: ...of revolving nosepiece or objective lens and engage the empty hole in the light path 4 Pull out the field iris diaphragm knob to close this iris and push in the aperture iris diaphragm knob to fully o...

Page 18: ...h If neither of these cubes is available move some other fluorescent cube into the light path 3 Screw the centering screen BH2 SGRF into the revolving nosepiece and move it into the light path 4 Push...

Page 19: ...filters with the marked side facing toward the observer As you insert a filter you will hear two clicks At the first the filter is at the empty position and at the second it enters the light path Not...

Page 20: ...505 BP450480 BP470490 BP420480 BP460 490 BP470490 BA515 BA515lF l FITC l Acridine orange DNA RNA l Auramine Band Pass Barrier Filter Combinations I I U U MNUA DM400 BP360 370 BA420 460 For observing o...

Page 21: ...Apo 20x 40x 40x01 ReSlecteti u v BV 0 0 0 0 0 0 0 0 0 0 0 0 Transtiittred Transmitted phase Nomarski contrast DIG 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Recommended combination Ox Usable but image may be dark...

Page 22: ...vation transmitted light phase E contrast observation and transmitted light differential interference contrast observation as well as reflected 2 light fluorescence observation With specimens th at fa...

Page 23: ...IC slider to adjust contrast of the differential interference m contrast image l 9 Move the cube corresponding to the desired excitation into the light path and open the light excluding shutter 2 10 A...

Page 24: ...00 a Wavelength nm Wavelength nm 2 U excitation cube Narrow band 5 BV excitation cube Narrow band 100 __ ___ ___ DM400 BA420 _______ DM455 1 I 40d I I I 600 I Wavelength nm 1 Waveilength nm 3 V excita...

Page 25: ...I I 600 I 600 800 3OC Wavelength nm Wavelength nm 8 B excitation cube Surer wide band II G excitation cube Wide band p iiiq p i q I I I I 7 I I I 400 600 800 Wavelength nm 100 I 8 I I 7 I 7 BA590 BP5...

Page 26: ...h nm Wavelength nm 14 IG excitation cube Wide band 17 IB excitation cube for color separation Wide band U MWlBAl 1001 s I I 8 I 8 I L 600 800 Wavelength nm Wavelength nm 15 IY excitation cube Wide ban...

Page 27: ...ercury Burner Spectrum 3651366 100 r s 312 6 313 2 300 400 500 600 700 800 Wavelength nm For fluorochrome emission a light beam having a specific wavelength is selected from a wide spectrum of wavelen...

Page 28: ...become possible by combining the universal reflected light vertical illuminator U URA with metallurgical UIS objectives brightfield cube U MBF or darkfield cube U MDF Power a lamp housing Objective BX...

Page 29: ...cal Illuminator shown on page 3 1 B Cube For reflected light observation with white light use a brightfield cube U MBF a darkfield cube U MDF and a differential interference contrast tube U MDIC etc 1...

Page 30: ...enon burner for darkfield observation illumination at the periphery may be slightly insufficient depending on the specimen If the brightfield cube U MBF is engaged while the mercury burner or xenon bu...

Page 31: ...etic index sticker I and affix it to the turret Fig 2 Use a sharp object such as the tip of a ballpoint pen or mechanical pencil to lift the cube s magnetic index sticker Move the magnetic cube index...

Page 32: ...e the bulb clamping levers to their original positions to secure the bulb Figs 6 7 Do not touch the bulb with bare hands If fingerprints or other smears are accidentally left on the bulb wipe it clean...

Page 33: ...place by the magnet on the underside of the vertical illuminator 3 Reattach the revolving nosepiece I Connect the lamp housing cord connector to the lamp housing connector I on the power supply unit 2...

Page 34: ...the revolving nosepiece to engage the 1OX objective then place a specimen on the stage and bring the image into approximate focus 2 Pull out the field iris diaphragm knob iJ on the universal fluoresc...

Page 35: ...rture of the illumination system to control the depth of focus contrast resolution etc 0 Reflected light darkfield observation Always keep the aperture iris diaphragm knob pushed in to leave the diaph...

Page 36: ...eld observation or if needed to shorten the exposure time during photomicrography or to brighten the field of view during darkfield observation remove the filter from the light path When using the DIC...

Page 37: ...select the interference color of the background and to achieve the maximum contrast depending on the specimen under observation as outlined below Fig 18 2 Rotating the prism control knob of the U DICR...

Page 38: ...erference Contrast Observation outlined on page 32 1 Place the specimen on the stage and move the stage to bring the specimen into focus Simple polarized light observation is now possible 2 Adjust the...

Page 39: ...0 80 22 5 21 0 12 0 10 6 3 4 2 58 1 34 0 84 0 67 0 42 50x 100x 200x 500x 1000x 70 1 18 4 6 09 2 50 0 87 4 4 2 2 1 1 0 44 0 22 75x 150x 300x 750x 1500x 75x 150x 300x 750x 1500x 52 2 13 7 4 64 2 03 0 72...

Page 40: ...the image circumscribes the field of view A cube unsuitable for the specimen is used Change to suitable cube The objectives are not inserted into the light Rotate the revolving nosepiece until it pat...

Page 41: ...lens of the objective is dirty Immersion oil is not being used with an oil immersion objective Recommended immersion oil not used DF insert not mounted The specimen is placed in a tilted position on t...

Page 42: ...ns Lamp Housing Collector lens focusing knob Not used with a halogen bulb Lamp Lamp up down adjustment knob I Lame focusina I PHILIPS 7724 long life type or Lamp left right PHILIPS 7023 high brightnes...

Page 43: ...off the main switch and wait for bulb lamp socket and lamp house to cool down Fig 21 Use only the 12V IOOW halogen lamp PHILIPS 7724 or 7023 Do not touch the halogen bulb with bare hands If the bulb...

Page 44: ...t circuit etc or in case of overcurrent When they activate the center portion of the breaker will pop up and the power will be cut off To reset the breakers press in the center portion If a breaker ac...

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