Olympus EHT-421, Instruction Manual

Page 1: ...OLYMPUS RESEARCH MICROSCOPES INSTRUCTION MANUAL MODELS FHT EHTWITH W ...

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Page 3: ...EARCH MICROSCOPES MODELS FHT EHT TABLE OF CONTENTS I STANDARD EQUIPMENT 2 II SPECiFiCATiONS 4 Ill IDENTIFICATION OF VARIOUS COMPONENTS 5 IV DESCRIPTION OF EACH COMPONENT 6 V OPERATING THE MICROSCOPE 14 VI OPTICAL CHARACTERiSTICS 19 ...

Page 4: ...n Total Model No Eyepieces Objectives Stage denser magni fication Bi P7x BiWF10x Bi P15x paired Ach 4x Ach lOx N A 28x FHT 531 Photo eyepieces Ach 40x 1 25 1500x FK2 5x FK3 3x Ach 100x oill FK5x FK6 7x leach Bi P7x Bi High Eye FrS point WF10x Bi P15x Ach4x Ach lOx Square FHT 532 Bi K20x paired FI40x Fll00x mechani N A 28x Photo eyepieces loill cal stage 1 40 2000x FK2 5x FK3 3x with left FK5x FK6 ...

Page 5: ...magni fication Bi P7x Bi WF lOx Bi P15x paired Ach 4x Ach lOx N A 28x EHT 431 Photo eyepieces Ach40x Achloox 1 25 1500x FK2 5x FK3 3x oill FK5x FK6 7x 1 each Bi P7x Bi High Eye pointWF10x Bi P15x Ach4x Ach lOx CrS VH EHT 432 Bi K20x paired FI 40x FI loox Square N A 28x Photo eyepieces oill mechani 1 40 2000x FK2 5x FK3 3x cal stage FK5x FK6 7x 1each with low Bi P7x Bi High Eye drive controls point...

Page 6: ... eyepiece tube Interpupillary distance adjustment from 56mm to 74mm with photo tube Light path selector lever FrS CrS VH Rotatable graduated mech Rotatable graduated mech Stages anical stage with horizontal anical stage with low drive drive controls movement controls movement range range 44mm x 76mm Ver 52mm x 76mm Vernier nier scales reading to O 1mm scales reading to 0 1mm Achromatic NA 1 40 wit...

Page 7: ...III IDENTIFICATION OF VARIOUS COMPONENTS Trinocular Observation Tube Limb Voltage Adjustment Knob FHT 533 5 Eyepiece Revolving Nosepiece Objective 5 Conden r Voltmeter ...

Page 8: ...Mount Light Exit Coane Adjustment Knob Fine Adjustment Knob 2 Condenser The condenser may be mounted on the condenser mount by first inserting the condenser into the condenser mount from below aligning the positioning dots on the condenser and condenser mount and then clamping with the clamping screw Condenser centration can be accomplished by means of two centering knobs Vertical movement of the ...

Page 9: ...aplanatic Condenser N A 1 4 Achromaticl aplanatic Condenser Condenser Clamping Screw Centering Knob Aperture Iris Diaphragm Cuntrol Aing r CD 4 Condenser N A 0 85 Condenser Condenser Mount Aperture Iris l _ Diaphragm Control lever Alignment Dots Condenser Mount Slide Clamping Screw Alignment Dots Slide Lever Condenser MOllnt Condenser Clamping Screw Centering Knob Filter Mount Alignment Dots Apert...

Page 10: ...is required however it is recommended to set it to position M or H according to objective magnification as in case with photomicrography Voltage Adjustment Knob Grounding terminal Line Voltage Adjustment Screw o Voltage Adjustment The minimum voltage required for the light source can be varied qv means of the line voltage adjustment screw provided at the back of the microscope base in accordance w...

Page 11: ...b in order to obtain optimum light intensity with no need to further proceed to the following procedure 3 3 Even after the voltage adjustment knob is turned on if the bulb does not light or lights up bright immediately after switching on rotate gradually the line voltage adjustment screw at the back of the base with a coin until the lamp dims o According to the fluctuations of line voltage and fre...

Page 12: ...fingerprints or stains on the bulb 5 Revolving Nosepiece The Quintuple revolving nosepiece rotates on ball bearings A knurled ring is provided for slip free and smooth rotation Each objective clicks Into position accurately maintaining proper optical alignment Also each objective hole is coded with the letters A 8 C D and E in order to indicate where the objectives should be mounted as A is for 4x...

Page 13: ...is obtained It is good practice to memorize the individual interpupillary distance setting A scale for this purpose is located between the eyepiece tubes The mechanical tube length of the SW observation tubes is 160mm when this scale is set at 62 l The eyepiece tubes are provided with diopter and tube length adjustment rings A light path selector lever to direct the light to observation tube or ph...

Page 14: ...n 44mm East west excursion 76mm Each control is provided with a scale 0 50 for Y excursion 50 120 for X excursion and a vernier reading to 0 1 mm Stage rotation can be clamped by a clamping screw The stage may be used as a plain stage by removing the specimen holder assembly Graduated Scale for North South Movement Stage Rotation Clamping Screw Graduated Scale for East West Movement Specimen Holde...

Page 15: ...rol Knob Specimen Holdel Clamping Screw for Stage Rotation l e f or Rotation r uat Scale for onh SolJthMovemeoJ Stage Clamping lever 3 Square Mechanical Stage with Low Drive Controls Cs VH This mechanical stage is operated by coaxial low drive controls on rack and pinion for north south and east west movements The working range of the specimen holder IS the same as that of the CrS VH 4 Square Coax...

Page 16: ...2 Diopter Adjustment a For FHT and EHT 1 Rotate the diopter ring on the right eyepiece tube to match the scale on the ring to your interpupillary distance setting which you obtained from scale CD as described in the preceding paragraph 1 121 2 Look at the image through the right eyepiece with your right eye and focus on the specimen with the fine adjustment knobs 3 Next look at the image through t...

Page 17: ...ield iris diaphragm rvvv IJU NUUU Before centration After centration 6 While widening the diameter of the field progressively use the con denser centering knobs to bring the diaphragm image into the center of the field of view If the polygonal image of the iris diaphragm becomes inscribed in the field it means that the field iris diaphragm is centered Slightly increase the diameter of the field ir...

Page 18: ...piece the field number will be varied which necessitates re adjustment of the diameter at the field diaphragm 2 Aperture Iris Diaphragm An aperture iris diaphragm opened too wide impairs image con trast due to internal reflections and related factors On the other hand if the diaphragm is stopped down exce 5ivcly rC50lution is unduly reduced It is therefore suggested to match the opening of the ape...

Page 19: ...ecimen 5 Turn the revolving nosepiece to bring the objective to be used into the light path F Use of Immersion Optical Components 1 Immersion Objectives 1 Focus on the specimen with a low power objective 21 Put a drop of immersion oil on both the specimen and the objective front lens 3 Turn the revolving nosepiece to bring the immersion objective into the light path and focus with the fine adjustm...

Page 20: ...Ving power decreases in this direction s 2 Procedure 1 Stop down the aperture iris diaphragm 2 Loosen the clamping screw and pull out the aperture diaphragm with the slide lever The direction of diaphragm displacement should be at right angles to the specimen detail to be observed For example jf it is desired to identify two parallel details very close to each other as two separate Jines the apert...

Page 21: ...epth 01 Foo on III I 7 8 0 8 0 8 7JO 7 8 0 bIIr T8 51 Fl lldolV_lmml 0 8 85 0 0 185 0 0 0 185 0 83 as 0 926 O Ies WF Tot MlgnofQ1iQr l 118 01 o th of Focus III I 114 1 77 9 7 s 0 7 114 1 27 0 7 neld of Vlew La 0 OA5 0 18 La 0 0 S PIS Total ilicatlon 800 800 111 51 eclth 01 foeus 1 JI1 131 4 21 0 2 0 0 31 4 21 0 7 0 Field of VIaw lmml 2 38 0 95 0 238 0 0 238 o 2 38 0 85 0 416 0238 000 TOtal tugnifi...

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