Olympus IX73, Instructions Manual

Page 1: ...odel IX73 To ensure the safety obtain optimum performance and to familiarize yourself fully with the use of this microscope we recommend that you study this manual thoroughly before operating the micr...

Page 2: ...cal and Electronic Equip ment this symbol indicates that the product must not be disposed of as unsorted municipal waste but should be collected separately Refer to your local Olympus distributor in E...

Page 3: ...ightness 14 2 Light Path Selection 15 3 Coded intermediate magnification changer IX3 CAS 15 4 Frame Fix Plate IX3 FP 15 5 Dust tray 16 4 2 Focusing Block 17 1 Rotation Direction of the Coarse Fine Adj...

Page 4: ...Adjustment Knob Tension 27 5 Condenser refocusing stopper 27 4 6 Condenser 28 1 Centering the Condenser 28 2 Using the Aperture Iris Diaphragm 30 4 7 Oil or Water Immersion Objective 31 1 Using Oil or...

Page 5: ...ation Separate Manual 44 5 5 Relief Contrast Observation Separate Manual 44 6 CAMERA RECORDING 45 1 Camera Adapter 45 2 Changing of Light Path 45 3 Selecting the Camera Adapter Magnification 45 4 Inst...

Page 6: ...e injury or damage to the equipment or other property It may also be used to alert against unsafe practices Indicates commentary for ease of operation and maintenance 6 Attaching the Stage 55 7 Attach...

Page 7: ...tter IX3 CBM U HSCBM Configuration and usage of the modules available in the IX73 motor ized system or the IX73 motorized coded system U LEDPS LED Power supply U CB5S Control Box for coded function Be...

Page 8: ...he liquid or water off if it is spilt on them 4 The microscope is not provided with a dust proof mechanism 5 Install the microscope on a sturdy level table or bench 6 When moving the microscope remove...

Page 9: ...e objective lens or the specu lar reflection light of the specimen 20 Be careful invisible wavelength light ultraviolet or infrared may be emitted depending on illumination methods 21 If the skin is e...

Page 10: ...product is applied Class A Some interference may occur if this system is used in domestic location USA CAUTION Federal law restricts this device to sale by or on the order of an appropriately licensed...

Page 11: ...light observation dark field observa tion observation with high magnification if the illumination light is not sufficient use the halogen lamp housing 8 If you use IX3 LHLEDC for observation with the...

Page 12: ...blower and gently wipe the lens or filter with a piece of cleaning paper or clean gauze For wiping fingerprints and oil stains use a piece of cleaning paper moistened with commercially available absol...

Page 13: ...u can configure the IX73 motorized coded system in combination with modules marked For details refer to IX3 CBM instruction manual Modules marked are components of the IX73 coded system For details re...

Page 14: ...justment scale p 23 Coarse adjustment knob tension adjustment ring p 17 Coarse adjustment knob p 17 Fine adjustment knob p 17 X axis knob p 21 Y axis knob p 21 Light intensity control knob p 14 Field...

Page 15: ...17 Motorized LWD condenser IX3 LWUCDA Motorized operation button TURRET Switches the turret of the condenser POL Switches IN OUT of the polarizer AS Opens the aperture iris diaphragm AS Narrows down t...

Page 16: ...PO Polarized light observation PH Phase contrast observation BF Brightfield observation FL Fluorescence observation OM1 to OM6 Buttons used to change the observation mode by connecting to PC and opera...

Page 17: ...L L For details refer to the separate instruction manual Manipulator mounting screw holes M6 screw x 6 IX MVR mounting screw holes On both sides Plain Stage IX2 SP Mechanical Stage IX MVR X axis knob...

Page 18: ...xis Adjust the brightness Start observation Controls Used Page 2 Lamp ON OFF button ON p 14 4 Filter pocket p 26 7 Filter pocket p 26 Main switch I ON p 14 3 Light intensity control knob p 14 Select t...

Page 19: ...13 IX73 Make a photocopy of the observation procedure pages and post it near your microscope a d e 1 2 3 4 5 7 8 b c 3 2 3 6 9...

Page 20: ...knob a on the power supply unit is defeated and only the light intensity control knob e on the front of the microscope is available Press the transmitted light ON OFF button f so that the LED is illum...

Page 21: ...e coded intermediate magnification changer IX3 CAS to switch the observation magnification in the following 3 levels ac cording to the objective lens magnification 1X 1 6X 2X For the assembly procedur...

Page 22: ...icroscope Remove and clean it on a regular basis Rotate the fixing screws 2 pcs to remove them Slide the dust tray to remove it Wash the dust tray with water and wipe it with the dry cloth to dry well...

Page 23: ...pre focusing lever d in the direction of the arrow to lock it Hereafter the upper limit of the coarse adjustment will be limited at the position where the lever is locked When bringing a specimen in f...

Page 24: ...ecimen may be popped out IX3 HOW Open the specimen holder b of IX3 HOW set the microplate in the center push it toward the right diagonal direction and return the specimen holder back to the original...

Page 25: ...dvance Do not tighten the fixing screws too firmly The dish may be damaged Set the fixing holder e in the center of IX3 HO35D g so that the cut out meets the holder fixing knob i Loosen the holder fix...

Page 26: ...al hold ers are available A Terasaki plate holder is available for holding Terasaki plates 72 well 60 well When using this it is necessary to replace the stage scales with those provided with the plat...

Page 27: ...chanical stage with right handle IX3 SVR To move the specimen to a desired position rotate the X axis knob a and Y axis knob b If the stage is used for a long time period the stage movement range may...

Page 28: ...ed position rotate the X axis knob and Y axis knob in the same manner as IX3 SVR The stage travel area is 130 mm X axis x 85 mm Y axis CAUTION With the Stage BX3 SSU or IX3 SVR A grounding wire can be...

Page 29: ...bjective Engage a low power objective e g 10X in the light path rotate only the right diopter adjustment ring a to bring the sample into focus At this time do not touch the coarse fine adjustment knob...

Page 30: ...a light uniform force or by applying the frame against a rubber sheet Do not grasp the frame with a strong force as this may deform the frame and make it harder to remove it Re attach the micrometer m...

Page 31: ...lting the illumination column upwards or downwards make sure not to catch your fingers in the hinge joint The tilt clamping screw should normally be tightened during use If the microscope is used whil...

Page 32: ...smudges on the filter surfaces Shortly after the transmitted light illumination has been used the filter will be very hot Be sure to set the main switch to OFF and allow the filter holder and filters...

Page 33: ...stment tighten the two knob clamping screws b se curely 1 2 3 A mechanism returns the condenser back to the original position easily after moving the condenser To use the condenser refocusing stopper...

Page 34: ...lever a install the Allen screwdriver provided with the microscope in the adjust ment hole b and rotate it so that the field iris diaphragm image is centered in the field of view of the eyepieces To...

Page 35: ...so that the field iris diaphragm image is centered in the field of view of the eyepieces Slowly move the condenser height fine adjustment knob toward the front to bring the field iris diaphragm image...

Page 36: ...ition may be improved by keeping the aperture iris diaphragm stopped down a little In general a good image is obtained if the diaphragm is stopped down to between 70 and 80 of the N A of the objective...

Page 37: ...image will be degraded Make sure the oil is free of air bubbles After use remove immersion oil from the objective front lens by wip ing with gauze slightly moistened with absolute alcohol The presenc...

Page 38: ...r equipped objective perform the following adjustment as required Adjustment Procedure If the cover glass thickness is known set the correction collar a to that value on the scale If the cover glass t...

Page 39: ...X3 LWUCDA Small Diameter PH1 PHL free or PH2 C free Large Diameter PH3 DIC40 DIC60 DIC100 With the IX ULWCD The IX PHCU or IX PH1U can be attached only in the Ph1 and PhC Do not remove the built in el...

Page 40: ...e contrast ring to be used to the turret hole by push ing it to the spring inside the turret Tighten the optical element fixing screw by using the condenser centering knobs Be careful not to apply pre...

Page 41: ...ded with each optical element in the index insertion hole having the same number h as the number of the position g in which the corresponding optical element is mount ed IX ULWCD does not have the ind...

Page 42: ...g screws in positions marked so that the ring slit image overlaps with the phase plate of the objective Do not release the hand suddenly while the optical element center ing knobs are being pushed in...

Page 43: ...0 IX2 DIC40HC IX2 DIC40HR 60XO IX2 DIC60 60XW IX2 DIC60 IX2 DIC60 60XS IX2 DIC60 IX2 DIC60 100XO IX2 DIC100 IX2 DIC100 100XOPH IX2 DIC100 IX2 DIC100 PLAPON 60XO IX2 DIC60 60XOPH IX2 DIC60 UPLFLN 10X2...

Page 44: ...e DIC prism s frame hits the bottom of the position and the prism s positioning pin fits into the pin hole When inserting push the spring c inside the turret slightly sideward Be careful not to touch...

Page 45: ...at the side with indication faces down insert it into the revolving nosepiece With the DIC Slider U DICTS U DICTHC U DICTHR If you use these sliders the analyzer U ANT cannot be used The transmitted D...

Page 46: ...on or simplified polarizer light observation Loosen the polarizer clamping screw g using the Allen screw driver Align the pin hole on the condenser with the positioning pin h on the condenser and inse...

Page 47: ...e knob is stopped First a black interference stripe then a rainbow colored interference stripe will be observed Stop the knob at the position in which the black interference stripe can be seen State o...

Page 48: ...With sensitive color observation using the U UCD8 engage the UUCDTP530 1 plate sensitive color plate in the light path Setting the background color to dark enables an observation like darkfield observ...

Page 49: ...used loosen the clamping of the polarizer rota tion clamping knob by rotating slightly the polarizer rotation clamp ing knob d in a counter clockwise direction Move the polarizer rotation clamping kn...

Page 50: ...Light Fluorescence Observation Separate Manual 5 5 Relief Contrast Observation Separate Manual Refer to the separate instruction manual REFLECTED FLUORESCENCE SYSTEM Refer to the separate instruction...

Page 51: ...t side port can be changed by using the light path selector lever on the side of the microscope page 15 Eyepiece Camera Left side port 100 0 50 50 0 100 If acquiring the image by setting the light pat...

Page 52: ...or in the camera port combining the intermedi ate tube IX ATU the trinocular tube U TR30 series 1 6X or 2X of the coded intermediate magnification changer IX3 CAS is being used High contrast specimen...

Page 53: ...a condenser that matches the objective 28 Field iris diaphragm is not properly centered Center the field iris diaphragm cor rectly 28 Field iris diaphragm is stopped down too far Open the field iris d...

Page 54: ...t and phase plate are not centered Center them correctly 36 A plastic culture vessel is used Replace the plastic culture vessel with a glass vessel 37 g A part or one side of the field of view is blur...

Page 55: ...Movement mechanism Ultrasonic drive Maximum speed 30 mm sec Single rough fine adjustment handle XY controller X and Y axis knob with adjust able tension Right long axis handle can be mounted by rever...

Page 56: ...e For the connection between cables and the control box refer to the instruction manual of the control box to be used 6 3 1 10 13 9 5 11 7 14 Terasaki plate holder IX HOT Slide glass holder IX HOS Pet...

Page 57: ...pins carefully from the above paying attention not to tilt the revolving nosepiece 2 CAUTION Tighten the revolving nosepiece fixing screws d 5 positions by using the Allen wrench provided with the mic...

Page 58: ...ow While pushing down the bulb clamping lever a hold the halogen bulb b with gloves or a piece of gauze insert the bulb pins c straight and fully into the pin position d on the lamp socket Then return...

Page 59: ...t the same procedure applies to the LED lamp house IX3 LHLEDC Loosen the two clamping screws a of the illumination column with the Allen screwdriver provided with the microscope Fit the lamp housing t...

Page 60: ...tray By using the provided Allen screwdriver loosen the fixing screws e 2 pcs of the deck where the fluorescence mirror turret is to be mounted until the fixing screws slightly come out from the screw...

Page 61: ...he revolving nosepiece is rotated Do not subject the stage center plate to impact or excessive force as this may deform it To facilitate confirmation of the objective front lens position and applicati...

Page 62: ...ectly place the Y axis scale i on the top of the stage s Y axis guide The scale will be held in place magnetically Attaching the substages CK2 SS These plates can be attached in the same way as outlin...

Page 63: ...ositions a in the nosepiece attach the dust caps provided After all objective lenses are mounted fit the stage center plate into the stage 1 CAUTION CAUTION 2 If the condenser refocusing stopper is at...

Page 64: ...the condenser turret should be positioned on the right hand side Push the UCD adapter horizontally into the condenser holder until the condenser s positioning pin fits into the positioning groove on t...

Page 65: ...again This lowers the eye point height by approxi mately 30 mm 1 2 Trinocular Tube U TR30 2 U TR30NIR U TR30H 2 First mount the IX ATU intermediate tube Using the Allen screwdriver loosen the observa...

Page 66: ...1 2 3 Twelve threaded holes a are provided on the microscope for mounting accessories such as a micromanipulator Insert the cylindrical material diameter less than 11 5 mm height higher than 5 mm betw...

Page 67: ...f the microscope CAUTION 1 Attaching the Fixing Plate Attach the fixing plate on the front side of the microscope and fix it by using a commercially available 5 mm Allen wrench 2 13 Using the Frame Fi...

Page 68: ...re connecting cables CAUTION 14 Connecting the Cables b a d c e f Connect the plug a of the lamp housing or illumination column to the connector b firmly Connect the hand switch plug or power supply c...

Page 69: ...he cable holders and the method of cable distribution are illustrated below If the cable holders are attached in widely deviated positions the cable may become unable to reach the connector Be sure to...

Page 70: ...scope is aligned with the notch of the cable cover Put the cables coming from the microscope through the cable cover Tighten the fixing knob to the microscope Put the cables coming from the modules at...

Page 71: ...ration of the warranty period If you have any questions please contact Olympus Check results Date Check items 1 More than 8 years have passed since original purchase or the total power ON time has exc...

Page 72: ...coupling Table 1 Certified Cord A power supply cord should be certified by one of the agencies listed in Table 1 or comprised of cordage marked with an agency marking per Table 1 or marked per Table 2...

Page 73: ...ic Cables BASEC BASEC HAR 10 10 30 N V KEMA KEMA KEUR HAR 10 30 30 SEMKO AB Svenska Elektriska Materielkontrollanstalter SEMKO HAR 10 10 50 sterreichischer Verband f r Elektrotechnik VE VE HAR 30 10 5...

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