VWR 630-3254, Instruction Manual

Page 1: ...Instruction manual VWR Microscope VisiScope Series 400 VERSION 02 ISSUED 03 2021 EU cat no 630 3254 630 3256 630 3255...

Page 2: ...CTURER Europe VWR International bv Researchpark Haasrode 2020 Geldenaaksebaan 464 B 3001 Leuven 32 16 385011 be vwr com UK Importer VWR International Ltd Hunter Boulevard Magna Park Lutterworth Leices...

Page 3: ...the coarse focus tension 19 10 4 Diopter adjustment 19 10 5 Adjusting interpupillary distance 20 10 6 Use of eyeshields 20 10 7 Selecting the light path 20 10 8 Stage and stage inserts 21 10 8 1 Insta...

Page 4: ...responsibility deriving from incorrect instrument use that does not comply with this manual 2 Safety Information Avoiding Electrical Shock Before plugging in the power supply make sure that the suppl...

Page 5: ...hase contrast slider 7 Glass insert for stage 8 Metal insert for stage 9 Objectives 10 Eyepieces 11 Stage inserts 12 Centering telescope 13 Green filter IF550 14 Lens cleaning tissue 15 UV shield 16 D...

Page 6: ...ondenser 3 LED illuminator 4 Objectives 5 Eyepieces 6 Anti glow cap 7 Glass insert for stage 8 Metal insert for stage 9 Mechanical stage 10 UV shield 11 Stage inserts 12 Lens cleaning tissue 13 Dust c...

Page 7: ...bjectives 6 Eyepieces 7 Glass insert for stage 8 Metal insert for stage 9 Mechanical stage 10 Stage inserts 11 Camera 12 C mount 13 Mouse 14 Keyboard 15 Monitor 16 UV shield 17 Power supply power cord...

Page 8: ...ds one around the neck and one around the base lift the microscope from the container and put it on a stable desk 5 Intended use For research and teaching use only Not intended for any animal or human...

Page 9: ...MENT RING EYEPIECE PHASE CONTRAST SLIDER LED HOUSING FILTER HOLDER COARSE FOCUS KNOB FINE FOCUS KNOB OBJECTIVE STAGE MICROSCOPE BODY LIGHT PATH SELECTOR LEVER STAGE PLATE CONDENSER PHOTO TV PORT FLUOR...

Page 10: ...EPIECE LED HOUSING FILTER HOLDER COARSE FOCUS KNOB FINE FOCUS KNOB OBJECTIVE STAGE MICROSCOPE BODY LIGHT PATH SELECTOR LEVER STAGE PLATE CONDENSER PHOTO TV PORT FLUORESCENCE CUBE LEVER TRANSMITTED LIG...

Page 11: ...G EYEPIECE FILTER HOLDER COARSE FOCUS KNOB FINE FOCUS KNOB OBJECTIVE STAGE MICROSCOPE BODY LIGHT PATH SELECTOR LEVER STAGE PLATE CONDENSER FLUORESCENCE CUBE LEVER TRANSMITTED LIGHT ADJUSTMENT KNOB REF...

Page 12: ...al 12 IT406 FL4D back side MONITOR POWER SUPPLY CABLE HDMI PLUG PC STICK HDMI CABLE TO MONITOR USB CABLE TO CAMERA MONITOR POWER SUPPLY CONNECTOR PC STICK POWER SUPPLY CABLE LED POWER SUPPLY CABLE USB...

Page 13: ...re very sensitive to dust To prevent dust and contamination from entering the microscope cover all the unused holes with dust caps 3 Fig 3 When operating use the low power objective 10X to search and...

Page 14: ...pieces into the tubes of the optical head Fig 6 8 5 Installing the condenser 1 Insert the condenser illumination unit into the bracket Fig 7 2 Turn the condenser illumination unit clockwise about 90 w...

Page 15: ...luminator No operation is therefore necessary Fig 10 8 7 Installing color filters Remove the filter holder then install the color filter you need Fig 11 12 1 Mount the color filter flat as shown in 1...

Page 16: ...eel 3 to lock the monitor Fig 14 8 9 Cable connection IT406 FL4D 1 Connect the USB mouse keyboard receiver in the slot A of the PC stick 2 Plug the USB3 0 cable in the slot B of the PC stick 3 Plug th...

Page 17: ...8 8 10 Connecting the power supply 1 Turn the main switch to O OFF before connecting the power supply 2 Insert the jack of the power supply into the power socket of the microscope Fig 19 3 Plug the po...

Page 18: ...pecimen Begin observation Adjust aperture diaphragm Insert in the light path the desired objective and focus the specimen Adjust interpupillary distance Adjust diopter Adjust brightness Main switch Ob...

Page 19: ...or the specimen defocuses while adjusting the fine focus knob 5 the coarse focus knob is too loose Turning the tension adjustment collar 4 in clockwise direction tightens the coarse focus tension 3 Ro...

Page 20: ...lasses Fold rubber eyeshields with both hands Folded eyeshields avoid scratching the lenses of eyeglasses Fig 25 Use without eyeglasses Raise eye shields and observe at the microscope placing eyes to...

Page 21: ...hand or by turning the knobs 1 of the mechanical stage Fig 28 When switching objectives take care not to touch the holder plates with the objectives as their weight may damage the front lens 10 8 1 In...

Page 22: ...e With low contrast specimens move the Aperture Diaphragm lever AS 1 to set the numerical aperture to about 70 80 of the objective s N A Fig 31 If necessary remove one eyepiece and looking into empty...

Page 23: ...via the two side bolts see chapter 10 3 4 The 4x 10x position 2 must be used with 4x and 10x phase contrast objectives the 20x 40x position 3 with the 20x and 40x and the SL position 4 is used for br...

Page 24: ...in the phase contrast slider using a screwdriver until the bright ring and the dark ring match Fig 38 4 The 4x and the 10X phase contrast objectives use the same ring on the phase contrast slider The...

Page 25: ...e microscope in fluorescence reflected light 13 1 Using the microscope Move the main switch in the position I to turn on the microscope then rotate the adjustment knob 1 Fig 40 to adjust the fluoresce...

Page 26: ...s provided with the fluorescence cube fix the new cube on the slider Fig 43 1 2 FIGURE 42 FIGURE 43 Filter name Excitation filter Dichroic mirror Barrier filter Applications VisiCube IT_B 325 375 nm 4...

Page 27: ...h rapid decay must first be observed in Fluorescence and then in Phase Contrast The combined observation allows you to easily identify some areas of the sample that emit fluorescence 1 Turn on the mic...

Page 28: ...apter 5 Connect the Reflex camera 4 to the T2 ring just installed Fig 49 6 Mount the other end of the relay tube 1 into the empty hole of the trinocular port then tighten the clamping screw Fig 47 T2...

Page 29: ...th the objective phase ring Adjust the condenser ring to match the objective phase ring The light ring and or the phase contrast ring is not centered Adjust the bolts to center them The objective used...

Page 30: ...n them For the best results use the VWR cleaning kit If you need to send the microscope to manufacturer for maintenance please use the original packaging 18 Technical service Web Resource Visit the VW...

Page 31: ...It is also your responsibility to decontaminate the equipment in case of biological chemical and or radiological contamination so as to protect from health hazards the persons involved in the disposa...

Page 32: ...14 85 00 international info fr vwr com 0 18 TTC min prix appel POLAND VWR International Sp z o o Limbowa 5 80 175 Gdansk Tel 48 58 32 38 200 info pl vwr com PORTUGAL VWR International Material de Labo...