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18 Feb 2011,<qsg-wf1> p. 1/8 

 

 

Q

UICK

S

TART 

G

UIDE

W

IDEFIELD 

Z

EISS 

A

XIOVERT 

200

 

/

 

V

OLOCITY

 

 

(SAFB

 

409)

 

 

 

Startup procedure ................................................................................................................ 2

 

Adjusting brightfield Köhler illumination ............................................................................... 2

 

Observing fluorescence through the eyepiece ..................................................................... 3

 

Acquiring images with the Improvision Volocity software ..................................................... 3

 

General informations ................................................................................................................................. 3

 

Acquiring the image ...................................................................................................................... 3

 

refocussing/ re-centring: ........................................................................................................................... 3

 

adjusting channel presets: ........................................................................................................................ 4

 

multidimensional acquisition setup: .......................................................................................................... 4

 

Shutdown procedure ............................................................................................................ 5

 

Important additional information ........................................................................................... 7

 

Volocity Video Toolbar .................................................................................................................. 7

 

Auto Exposure .............................................................................................................................. 8

 

Auto Contrast ................................................................................................................................ 8

 

Keypad controls: ........................................................................................................................... 8

 

Control buttons on the lefthand side of the microscope: ............................................................... 8

 

File formats ................................................................................................................................... 8

 

merge colours: .............................................................................................................................. 8

 

 

Facility for Imaging
by Light Microscopy

Observing Life As It Happens

Imperial College

London

Summary of Contents for Axiovert 200

Page 1: ...are 3 General informations 3 Acquiring the image 3 refocussing re centring 3 adjusting channel presets 4 multidimensional acquisition setup 4 Shutdown procedure 5 Important additional information 7 Vo...

Page 2: ...on with the joystick holding the button on top of the joystick makes the stage moving faster focus on your sample if focus beeps override the top limit by pushing the top focus button on the right sid...

Page 3: ...ters filters focus except the light path BINOCULAR FRONT PORT BASE PORT and the objectives Acquiring the image start the VOLOCITY software make sure the dongle is attached to a USB port click on the c...

Page 4: ...open Acquisition Setup dialogue o tick Change channels o add as many channels as you need with the button o from the appearing drop down menus choose the desired channel presets o if you don t want to...

Page 5: ...tage menu in the Acquisition Setup dialogue set Change XY using to Ludl XY Stage o handmade positioning Video menu Live Video Stage menu Clear All Points move to desired point Stage menu Add Point wit...

Page 6: ...meone s booked within two hours update usage in Sharepoint remove your samples clean objective lenses with fresh lens tissue and close incubation chamber clear up the desk save files onto the server o...

Page 7: ...18 Feb 2011 qsg wf1 p 7 8 Important additional information Volocity Video Toolbar...

Page 8: ...rd Control buttons on the lefthand side of the microscope lowest button switches between frontport empty eyepiece baseport camera middle button switches between sideport 100 empty 50 50 eye front base...

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